A histology-based atlas of the C57BL/6J mouse brain deformably registered to in vivo MRI for localized radiation and surgical targeting.
A histology-based atlas of the C57BL/6J mouse brain deformably registered to in vivo MRI for localized radiation and surgical targeting.
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DOI：
10.1088/0031-9155/54/24/005
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发表时间：
2009-12-21
影响因子：
3.5
通讯作者：
Ford E
中科院分区：
文献类型：
--
作者：
Purger D;McNutt T;Achanta P;Quiñones-Hinojosa A;Wong J;Ford E
C57BL/6J实验小鼠是神经生物学研究中常用的小鼠。C57BL/6J脑的数字图谱已被用于可视化、遗传表型和形态测定，但目前缺乏准确计算个体小鼠之间差异的能力。基于《the Mouse brain in Stereotaxic Coordinates 2nd edition》（San Diego, CA: Academic）的组织学图谱，我们开发了C57BL/6J脑的全三维数字图谱。地图集使用三角形网格来表示各种结构。图谱结构可以覆盖和变形到单个小鼠MR图像。在这项研究中，我们从组织学图谱中选择了18个结构。通过计算相应单个网格顶点的平均三维位置，可以为任何一组感兴趣的小鼠创建平均地图集。为了验证图谱的准确性，我们对5、8和9周大的三个年龄组的13只小鼠进行了侧脑室的形变登记。将单个小鼠侧脑室结构与相应的平均结构和原始组织学结构进行比较。我们发现，使用我们的方法创建的平均结构比单独基于组织学的图谱更准确地代表个体解剖，左侧脑室的平均顶点偏差为0.044 mm，而右侧脑室的平均顶点偏差为0.045 mm，而右侧脑室的平均顶点偏差为0.068 mm。我们的地图集表示为感兴趣的结构提供了直接的空间偏差。我们的研究结果表明，基于mri可变形组织学的图谱代表了一种精确的方法，可以获得小鼠群体的精确形态测量，并且这种方法可以应用于未来的表型实验以及外科手术或放射治疗的精确靶向。
The C57BL/6J laboratory mouse is commonly used in neurobiological research. Digital atlases of the C57BL/6J brain have been used for visualization, genetic phenotyping and morphometry, but currently lack the ability to accurately calculate deviations between individual mice. We developed a fully three-dimensional digital atlas of the C57BL/6J brain based on the histology atlas of The Mouse Brain in Stereotaxic Coordinates 2nd edn (San Diego, CA: Academic)). The atlas uses triangular meshes to represent the various structures. The atlas structures can be overlaid and deformed to individual mouse MR images. For this study, we selected 18 structures from the histological atlas. Average atlases can be created for any group of mice of interest by calculating the mean three-dimensional positions of corresponding individual mesh vertices. As a validation of the atlas’ accuracy, we performed deformable registration of the lateral ventricles to 13 MR brain scans of mice in three age groups: 5, 8 and 9 weeks old. Lateral ventricle structures from individual mice were compared to the corresponding average structures and the original histology structures. We found that the average structures created using our method more accurately represent individual anatomy than histology-based atlases alone, with mean vertex deviations of 0.044 mm versus 0.082 mm for the left lateral ventricle and 0.045 mm versus 0.068 mm for the right lateral ventricle. Our atlas representation gives direct spatial deviations for structures of interest. Our results indicate that MR-deformable histology-based atlases represent an accurate method to obtain accurate morphometric measurements of a population of mice, and that this method may be applied to phenotyping experiments in the future as well as precision targeting of surgical procedures or radiation treatment.
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影响因子：
3.8
作者：
Graves, Edward E.;Zhou, Hu;Boyer, Arthur L.
通讯作者：
Boyer, Arthur L.
DOI：
10.1109/34.121791
发表时间：
1992-02-01
影响因子：
23.6
作者：
BESL, PJ;MCKAY, ND
通讯作者：
MCKAY, ND
影响因子：
3
作者：
Fricke, ST;Vink, R;Faden, AI
通讯作者：
Faden, AI
影响因子：
5.3
作者：
Bock, NA;Kovacevic, N;Henkelman, RM
通讯作者：
Henkelman, RM
影响因子：
3.3
作者：
Ma, Y;Hof, PR;Benveniste, H
通讯作者：
Benveniste, H