Qualitative and quantitative differences between taste buds of the rat and mouse.
Qualitative and quantitative differences between taste buds of the rat and mouse.
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DOI：
10.1186/1471-2202-8-5
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发表时间：
2007-01-05
期刊：
影响因子：
2.4
通讯作者：
Kinnamon JC
中科院分区：
文献类型：
--
作者：
Ma H;Yang R;Thomas SM;Kinnamon JC
许多啮齿动物味蕾的电生理，超微结构和免疫细胞化学研究已经进行了大鼠味蕾。然而，近年来，小鼠已成为味蕾感觉传导的分子和其他研究的首选物种。大鼠和小鼠的味蕾有相同的细胞类型、感觉传导标记和突触蛋白吗？在本研究中，我们使用了针对PLCβ2、α-味蛋白、5-羟色胺（5-HT）、PGP 9.5和突触泡蛋白-2的抗血清，以确定两种啮齿动物味蕾中表达这些标记物的味细胞的百分比。我们还测定了味蕾中的味细胞数量以及味蕾体积。在小鼠和大鼠味蕾之间，对所有五种标记物显示免疫反应性的味细胞的百分比存在显著差异（p < 0.05）。大鼠味蕾对PLCβ2（31.8% vs 19.6%）、α-味蛋白（18% vs 14.6%）和突触泡蛋白-2（31.2% vs 26.3%）的免疫反应性显著高于小鼠。然而，小鼠有更多的细胞显示对5-HT（15.9%对13.7%）和PGP 9.5（14.3%对9.4%）的免疫反应性。小鼠味蕾平均含有85.8个味细胞，而大鼠味蕾平均含有68.4个味细胞。小鼠味蕾的平均体积（42，000 μm3）小于大鼠味蕾（64，200 μm3）。小鼠轮廓味蕾中味细胞的数密度（2.1个/1000 μm3）显著高于大鼠（1.2个/1000 μm3）。这些结果表明，大鼠和小鼠在表达信号分子的味觉细胞的百分比方面存在显着差异。我们推测，这些观察到的差异可能反映了他们的味觉处理的差异。
Numerous electrophysiological, ultrastructural, and immunocytochemical studies on rodent taste buds have been carried out on rat taste buds. In recent years, however, the mouse has become the species of choice for molecular and other studies on sensory transduction in taste buds. Do rat and mouse taste buds have the same cell types, sensory transduction markers and synaptic proteins? In the present study we have used antisera directed against PLCβ2, α-gustducin, serotonin (5-HT), PGP 9.5 and synaptobrevin-2 to determine the percentages of taste cells expressing these markers in taste buds in both rodent species. We also determined the numbers of taste cells in the taste buds as well as taste bud volume. There are significant differences (p < 0.05) between mouse and rat taste buds in the percentages of taste cells displaying immunoreactivity for all five markers. Rat taste buds display significantly more immunoreactivity than mice for PLCβ2 (31.8% vs 19.6%), α-gustducin (18% vs 14.6%), and synaptobrevin-2 (31.2% vs 26.3%). Mice, however, have more cells that display immunoreactivity to 5-HT (15.9% vs 13.7%) and PGP 9.5 (14.3% vs 9.4%). Mouse taste buds contain an average of 85.8 taste cells vs 68.4 taste cells in rat taste buds. The average volume of a mouse taste bud (42,000 μm3) is smaller than a rat taste bud (64,200 μm3). The numerical density of taste cells in mouse circumvallate taste buds (2.1 cells/1000 μm3) is significantly higher than that in the rat (1.2 cells/1000 μm3). These results suggest that rats and mice differ significantly in the percentages of taste cells expressing signaling molecules. We speculate that these observed dissimilarities may reflect differences in their gustatory processing.
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影响因子：
2.5
作者：
Clapp, TR;Yang, RB;Kinnamon, JC
通讯作者：
Kinnamon, JC
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3.5
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通讯作者：
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作者：
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通讯作者：
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影响因子：
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作者：
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