L. plantarum prevents enteroinvasive Escherichia coli-induced tight junction proteins changes in intestinal epithelial cells.
L. plantarum prevents enteroinvasive Escherichia coli-induced tight junction proteins changes in intestinal epithelial cells.
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植物乳杆菌可防止肠侵袭性大肠杆菌诱导的肠上皮细胞紧密连接蛋白变化
DOI：
10.1186/1471-2180-9-63
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发表时间：
2009-03-31
期刊：
影响因子：
4.2
通讯作者：
Jiang Y
中科院分区：
文献类型：
--
作者：
Qin H;Zhang Z;Hang X;Jiang Y
植物乳杆菌（Lactobacillus plantarum，L. plantarum）具有通过防止宿主细胞形态学、附着/消除（A/E）损伤形成、单层抗性和大分子渗透性的变化来保护免于肠致病性大肠杆菌（EPEC）诱导的上皮单层屏障功能的损伤的能力。然而，这种保护作用所涉及的细胞机制仍有待澄清。本研究旨在探讨L.目的观察植物乳杆菌对肠侵袭性大肠杆菌（EIEC）感染Caco-2细胞的影响，以及EIEC感染和植物乳杆菌粘附后Caco-2细胞单层通透性和葡聚糖通透性的变化，以及紧密连接蛋白Claudin-1、Occludin、JAM-1和ZO-1的分布和表达。采用激光共聚焦显微镜、免疫组化和Western blotting技术，用荧光素标记鬼笔环肽（FITC-phalloidin）对感染后的植物病原菌进行细胞骨架蛋白F-actin的观察。本研究表明，EIEC感染后，跨上皮电阻（TER）随时间推移而降低，葡聚糖积分强度（DII）随时间推移而升高，而L。与EIEC组比较，TER和DII的变化明显改善。L. plantarum可预防EIEC诱导的Claudin-1、Occludin、JAM-1和ZO-1蛋白表达和重排的损伤，并可减轻EIEC对细胞骨架蛋白F-actin的损伤。L. plantarum对EIEC感染引起的Caco-2单层细胞完整性的损伤及TJ蛋白的结构和分布具有保护作用。
It is increasingly recognized that Lactobacillus plantarum (L. plantarum) has the ability to protect against Enteropathogenic Escherichia coli (EPEC)-induced damage of the epithelial monolayer barrier function by preventing changes in host cell morphology, attaching/effacing (A/E) lesion formation, monolayer resistance, and macromolecular permeability. However, the cellular mechanism involved in this protective effect still remained to be clarified. This study was to investigate the effect of L. plantarum on the changes of Caco-2 cells responding to Enteroinvasive Escherichia coli (EIEC), the permeability of cell monolayer and the transmissivity of dextran, and the distribution and expression of the tight junction (TJ) proteins, such as Claudin-1, Occludin, JAM-1 and ZO-1 were examined when infected with EIEC or adhesived of L. plantarum after infection by confocal laser scanning microscopy (CLSM), immunohistochemistry and Western blotting, the cytoskeleton protein F-actin were observed with FITC-phalloidin. This study demonstrated that the transepithelial electrical resistance (TER) step down and dextran integrated intensity (DII) step up with time after infected with EIEC, but after treating with L. plantarum, the changes of TER and DII were improved as compared with EIEC group. L. plantarum prevented the damage of expression and rearrangement of Claudin-1, Occludin, JAM-1 and ZO-1 proteins induced by EIEC, and could ameliorate the injury of cytoskeleton protein F-actin infected with EIEC. L. plantarum exerted a protective effect against the damage to integrity of Caco-2 monolayer cells and the structure and distribution of TJ proteins by EIEC infection.
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影响因子：
3.7
作者：
Huebner, Elizabeth S.;Surawicz, Christina M.
通讯作者：
Surawicz, Christina M.
影响因子：
2.6
作者：
Parassol, N;Freitas, M;Rampal, P
通讯作者：
Rampal, P
影响因子：
5.5
作者：
Colegio, OR;Van Itallie, C;Anderson, JM
通讯作者：
Anderson, JM
影响因子：
--
作者：
Takahashi, M;Taguchi, H;Kamiya, S
通讯作者：
Kamiya, S
DOI：
10.1152/ajpgi.00341.2003
发表时间：
2004-04-01
影响因子：
4.5
作者：
Otte, JM;Podolsky, DK
通讯作者：
Podolsky, DK