Molecular mapping to species level of the tonsillar crypt microbiota associated with health and recurrent tonsillitis.
Molecular mapping to species level of the tonsillar crypt microbiota associated with health and recurrent tonsillitis.
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DOI：
10.1371/journal.pone.0056418
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kilian M
中科院分区：
文献类型：
--
作者：
Jensen A;Fagö-Olsen H;Sørensen CH;Kilian M
人腭扁桃体是粘膜免疫系统的中心抗原处理部位，经常受到急性和复发性感染的影响。这项研究比较了受复发性扁桃体炎影响的儿童和成人的扁桃体隐窝微生物群与健康成人和扁桃体增生儿童的微生物群。一种深入的基于16 S rRNA基因的焦磷酸测序方法与一种新的策略相结合，包括系统发育分析和物种特异性序列特征的检测，使微生物群的主要部分能够识别到物种水平。在儿童和成人中，都发现了由42至110个分类群组成的复杂微生物群。这包括在所有样本中发现的12个丰富属的核心微生物组，无论年龄和健康状况如何。然而，流感嗜血杆菌，奈瑟菌属，肺炎链球菌几乎只在儿童中检测到。相比之下，所有样品中均存在假乳链球菌。专性厌氧菌如卟啉单胞菌、普雷沃菌和梭杆菌在儿童中大量存在，但卟啉单胞菌和普雷沃菌的物种多样性在成人中更大，包括被认为是牙周病的假定病原体的物种，即牙龈卟啉单胞菌、牙髓卟啉单胞菌和嗜酸坦纳氏菌。Unifrac分析表明，复发性扁桃体炎与扁桃体隐窝微生物群的变化有关。坏死梭杆菌、中间链球菌和产黑色素普雷沃菌/组织滴虫与成人复发性扁桃体炎相关，而传统上与急性扁桃体炎相关的菌种如化脓性链球菌和金黄色葡萄球菌则很少。研究结果表明，复发性扁桃体炎是一种多微生物感染，其中财团内的相互作用的分类群发挥病因作用。该研究有助于人类微生物组数据，了解影响扁桃体的感染病因，并为进一步了解扁桃体中发生的强烈微生物-宿主相互作用的后果奠定了基础。
The human palatine tonsils, which belong to the central antigen handling sites of the mucosal immune system, are frequently affected by acute and recurrent infections. This study compared the microbiota of the tonsillar crypts in children and adults affected by recurrent tonsillitis with that of healthy adults and children with tonsillar hyperplasia. An in-depth 16S rRNA gene based pyrosequencing approach combined with a novel strategy that included phylogenetic analysis and detection of species-specific sequence signatures enabled identification of the major part of the microbiota to species level. A complex microbiota consisting of between 42 and 110 taxa was demonstrated in both children and adults. This included a core microbiome of 12 abundant genera found in all samples regardless of age and health status. Yet, Haemophilus influenzae, Neisseria species, and Streptococcus pneumoniae were almost exclusively detected in children. In contrast, Streptococcus pseudopneumoniae was present in all samples. Obligate anaerobes like Porphyromonas, Prevotella, and Fusobacterium were abundantly present in children, but the species diversity of Porphyromonas and Prevotella was larger in adults and included species that are considered putative pathogens in periodontal diseases, i.e. Porphyromonas gingivalis, Porphyromonas endodontalis, and Tannerella forsythia. Unifrac analysis showed that recurrent tonsillitis is associated with a shift in the microbiota of the tonsillar crypts. Fusobacterium necrophorum, Streptococcus intermedius and Prevotella melaninogenica/histicola were associated with recurrent tonsillitis in adults, whereas species traditionally associated with acute tonsillitis like pyogenic streptococci and Staphylococcus aureus were scarce. The findings suggest that recurrent tonsillitis is a polymicrobial infection in which interactions within consortia of taxa play an etiologic role. The study contributes to the human microbiome data, to the understanding of the etiology of infections affecting the tonsils, and forms a basis for further insight into the consequences of the intense microbe-host interactions that take place in the tonsils.
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影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
影响因子：
14.9
作者：
Claesson MJ;Wang Q;O'Sullivan O;Greene-Diniz R;Cole JR;Ross RP;O'Toole PW
通讯作者：
O'Toole PW
DOI：
10.1038/ismej.2010.30
发表时间：
2010-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1016/j.ijporl.2004.08.007
发表时间：
2005-01-01
影响因子：
1.5
作者：
Brook, I
通讯作者：
Brook, I
影响因子：
2.6
作者：
BROOK, I;FOOTE, PA
通讯作者：
FOOTE, PA