Golgi/plastid-type manganese superoxide dismutase involved in heat-stress tolerance during grain filling of rice.
Golgi/plastid-type manganese superoxide dismutase involved in heat-stress tolerance during grain filling of rice.
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DOI：
10.1111/pbi.12314
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发表时间：
2015-12
影响因子：
13.8
通讯作者：
Mitsui T
中科院分区：
文献类型：
--
作者：
Shiraya T;Mori T;Maruyama T;Sasaki M;Takamatsu T;Oikawa K;Itoh K;Kaneko K;Ichikawa H;Mitsui T
超氧化物歧化酶(SOD)被广泛认为在环境应激引起的活性氧物种的解毒中发挥作用。我们在水稻耐热胁迫品种中发现了锰超氧化物歧化酶1(MSD1)的特征表达。推导的氨基酸序列包含一个信号序列和一个N-糖基化位点。对瞬时表达MSD1-YFP的水稻和洋葱细胞的共聚焦成像分析表明，MSD1-YFP存在于高尔基体和叶绿体中，表明MSD1是一种独特的高尔基/叶绿体类型的超氧化物歧化酶。为了评估MSD1在耐热胁迫中的作用，我们获得了结构性高表达或抑制MSD1的转基因水稻植株。在33/28℃、12/12h生长条件下，MSD1结构性高表达水稻的稻米品质显著好于野生型。相反，MSD1基因敲除的水稻对热胁迫非常敏感。定量蛋白质组学分析表明，在高温胁迫下，MSD1的过表达上调了水稻籽粒中的活性氧清除系统、伴侣系统和品质控制系统。我们认为高尔基体MSD1在热胁迫适应中起着重要作用。
Superoxide dismutase (SOD) is widely assumed to play a role in the detoxification of reactive oxygen species caused by environmental stresses. We found a characteristic expression of manganese SOD 1 (MSD1) in a heat‐stress‐tolerant cultivar of rice (Oryza sativa). The deduced amino acid sequence contains a signal sequence and an N‐glycosylation site. Confocal imaging analysis of rice and onion cells transiently expressing MSD1‐YFP showed MSD1‐YFP in the Golgi apparatus and plastids, indicating that MSD1 is a unique Golgi/plastid‐type SOD. To evaluate the involvement of MSD1 in heat‐stress tolerance, we generated transgenic rice plants with either constitutive high expression or suppression of MSD1. The grain quality of rice with constitutive high expression of MSD1 grown at 33/28 °C, 12/12 h, was significantly better than that of the wild type. In contrast, MSD1‐knock‐down rice was markedly susceptible to heat stress. Quantitative shotgun proteomic analysis indicated that the overexpression of MSD1 up‐regulated reactive oxygen scavenging, chaperone and quality control systems in rice grains under heat stress. We propose that the Golgi/plastid MSD1 plays an important role in adaptation to heat stress.
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影响因子：
6.9
作者：
del Río, LA;Sandalio, LM;Zilinskas, BA
通讯作者：
Zilinskas, BA
DOI：
10.1270/jsbbr.10.91
发表时间：
2008-01-01
期刊：
Breeding Research
影响因子：
--
作者：
Ebitani, T.;Yamamoto, Y.;Funane, M.
通讯作者：
Funane, M.
影响因子：
5.1
作者：
CORNEJO, MJ;LUTH, D;BLECHL, AE
通讯作者：
BLECHL, AE
影响因子：
1.1
作者：
Azwan Awang, Azwan Awang;Rafiah Karim, Rafiah Karim;Mitsui, T.
通讯作者：
Mitsui, T.
影响因子：
5.4
作者：
He, P;Li, SG;Zhu, LH
通讯作者：
Zhu, LH