Coexistence of Three Dominant Bacterial Symbionts in a Social Aphid and Implications for Ecological Adaptation.
Coexistence of Three Dominant Bacterial Symbionts in a Social Aphid and Implications for Ecological Adaptation.
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在社会蚜虫中，三个主要的细菌共生体的共存及其对生态适应的影响。
DOI：
10.3390/insects12050416
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发表时间：
2021-05-06
期刊：
影响因子：
3
通讯作者：
Huang X
中科院分区：
文献类型：
--
作者：
Liu Q;Zhang H;Zeng L;Yu Y;Lin X;Huang X
大多数昆虫与各种共生体有关，这些共生体在昆虫生活史中起着至关重要的作用。蚜虫与共生体的共生关系是研究昆虫与共生体相互作用的一个很好的模式系统。竹黄蚜是亚热带地区典型的群居性蚜虫，在竹子上全年单性繁殖，是唯一专食硬竹秆的蚜虫。在这项研究中，我们调查了共生细菌群落与P.bambucicola。结果表明，竹栖青霉菌群多样性较低，但主要内共生菌Buchnera和2种次级内共生菌Pectobacterium和Wolbachia稳定共存，侵染率较高。结合竹生单胞菌的生物学特性，推测果胶杆菌可能帮助竹生单胞菌取食竹秆，沃尔巴克氏体可能调节竹生单胞菌有性生殖的丧失或具有营养作用。这些发现将推进我们对社会性蚜虫微生物组的认识，并为进一步研究竹生青霉共生体的功能作用奠定基础。蚜虫与一系列共生体有关，这些共生体对其宿主具有不同的生态和进化影响。迄今为止，大多数蚜虫物种的共生体群落特征仍然很差，特别是社会性蚜虫。在这项研究中，高通量16S rDNA扩增测序被用来评估社会蚜虫Pseudoregma bambucicola的细菌群落，并在蚂蚁出席和时间序列的细菌多样性的差异进行了评估。结果表明，竹黄青霉共生菌多样性较低，优势共生菌Buchnera、Pectobacterium和Wolbachia稳定共存。果胶杆菌可能帮助竹原甲取食硬竹茎，遗传距离分析表明竹原甲体内的果胶杆菌可能是一种新的共生菌。Wolbachia可能与竹荪生殖方式的转变有关，或具有营养作用。对竹节虫体内细菌群落多样性的统计检验表明，有蚂蚁参与的蚜虫种群的均匀度通常显著高于无蚂蚁参与的种群，但不同季节的蚜虫种群间差异不显著。
Most insects are associated with a variety of symbionts that play a crucial role in insect life history. Symbiosis of aphids and their symbionts is a good model system to study insect–symbiont interactions. Pseudoregma bambucicola is a typical social aphid that lives parthenogenetically throughout the year on bamboos in subtropical areas, and it is the only aphid that exclusively feeds on the hard stalks of bamboo. In this study, we surveyed the symbiotic bacterial community associated with P. bambucicola. Our results showed that the diversity of P. bambucicola microbiome was low, but three symbionts, namely the primary endosymbiont Buchnera and two secondary symbionts (Pectobacterium and Wolbachia), were stable coexisting with a high infection rate. Combined with the biology of P. bambucicola, we speculate that Pectobacterium may help P. bambucicola feed on the stalks of bamboo, and Wolbachia may regulate the loss of sexual reproduction or has a nutritional role in P. bambucicola. These findings will advance our knowledge of the microbiomes of social aphids and set the foundation for further studies on the functional roles of P. bambucicola symbionts. Aphids are associated with an array of symbionts that have diverse ecological and evolutionary effects on their hosts. To date, symbiont communities of most aphid species are still poorly characterized, especially for the social aphids. In this study, high-throughput 16S rDNA amplicon sequencing was used to assess the bacterial communities of the social aphid Pseudoregma bambucicola, and the differences in bacterial diversity with respect to ant attendance and time series were also assessed. We found that the diversity of symbionts in P. bambucicola was low and three dominant symbionts (Buchnera, Pectobacterium and Wolbachia) were stably coexisting. Pectobacterium may help P. bambucicola feed on the hard bamboo stems, and genetic distance analysis suggests that the Pectobacterium in P. bambucicola may be a new symbiont species. Wolbachia may be associated with the transition of reproduction mode or has a nutritional role in P. bambucicola. Statistical tests on the diversity of bacterial communities in P. bambucicola suggest that aphid populations attended by ants usually have a significantly higher evenness than populations without ant attendance but there was no significant difference among aphid populations from different seasons.
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影响因子：
3.2
作者：
Gómez-Valero, L;Soriano-Navarro, M;Latorre, A
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
1.7
作者：
Feng, Hong-Qiang;Zhao, Xin-Cheng;Guo, Yu-Yuan
通讯作者：
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影响因子：
15.5
作者：
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通讯作者：
Massart S
影响因子：
3.7
作者：
Gauthier, Jean-Pierre;Outreman, Yannick;Simon, Jean-Christophe
通讯作者：
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