Identification of differentially expressed proteins and phosphorylated proteins in rice seedlings in response to strigolactone treatment.
Identification of differentially expressed proteins and phosphorylated proteins in rice seedlings in response to strigolactone treatment.
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独脚金内酯处理下水稻幼苗差异表达蛋白和磷酸化蛋白的鉴定
DOI：
10.1371/journal.pone.0093947
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Chen F;Jiang L;Zheng J;Huang R;Wang H;Hong Z;Huang Y
独脚金内酯（Strigolactones，SL）是近年来发现的一种植物激素，具有抑制植物枝条分枝、调节植物生长发育及与寄生虫相互作用等功能。已有研究表明，植物D10蛋白是一个类胡萝卜素裂解双加氧酶，在SL生物合成中发挥作用。本研究利用水稻（Oryza sativa L.）spp. indica）来研究响应SL处理的蛋白质。当生长在黑暗中，d10突变体幼苗表现出细长的中胚轴，可以拯救通过外源性应用SL。可溶性蛋白质提取物制备d10突变体幼苗生长在黑暗中的GR 24，合成的SL类似物的存在下。在二维凝胶上分离可溶性蛋白并进行蛋白质组学分析。鉴定了差异表达的蛋白质和磷酸化状态响应于GR24处理而改变的磷蛋白。8个蛋白被发现是诱导或下调GR24，和一组不同的8磷蛋白被证明改变其磷酸化强度在黑暗中生长的d10幼苗响应GR24处理。对这些蛋白质的分析表明，它们是碳水化合物和氨基酸代谢途径的重要酶，也是细胞能量产生机制的关键组分。这些蛋白质可能代表SL信号通路的潜在靶点。本研究为SL控制植物分枝和发育的复杂负调控机制提供了新的见解。
Strigolactones (SLs) are recently identified plant hormones that inhibit shoot branching and control various aspects of plant growth, development and interaction with parasites. Previous studies have shown that plant D10 protein is a carotenoid cleavage dioxygenase that functions in SL biosynthesis. In this work, we used an allelic SL-deficient d10 mutant XJC of rice (Oryza sativa L. spp. indica) to investigate proteins that were responsive to SL treatment. When grown in darkness, d10 mutant seedlings exhibited elongated mesocotyl that could be rescued by exogenous application of SLs. Soluble protein extracts were prepared from d10 mutant seedlings grown in darkness in the presence of GR24, a synthetic SL analog. Soluble proteins were separated on two-dimensional gels and subjected to proteomic analysis. Proteins that were expressed differentially and phosphoproteins whose phosphorylation status changed in response to GR24 treatment were identified. Eight proteins were found to be induced or down-regulated by GR24, and a different set of 8 phosphoproteins were shown to change their phosphorylation intensities in the dark-grown d10 seedlings in response to GR24 treatment. Analysis of these proteins revealed that they are important enzymes of the carbohydrate and amino acid metabolic pathways and key components of the cellular energy generation machinery. These proteins may represent potential targets of the SL signaling pathway. This study provides new insight into the complex and negative regulatory mechanism by which SLs control shoot branching and plant development.
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