Development of an efficient recombinant mosquito densovirus-mediated RNA interference system and its preliminary application in mosquito control.
Development of an efficient recombinant mosquito densovirus-mediated RNA interference system and its preliminary application in mosquito control.
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高效重组蚊浓核病毒介导的RNA干扰系统的研制及其在灭蚊中的初步应用
DOI：
10.1371/journal.pone.0021329
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Gu J;Liu M;Deng Y;Peng H;Chen X
埃及伊蚊浓核病毒（AeDNV）具有作为将外源核酸递送到蚊子细胞中的载体的潜力。在本研究中，我们研究了含有重组病毒转导基因组的质粒在C6/C36细胞中诱导RNA干扰（RNAi）效应的能力。然后，我们评估了重组AeDNV载体在白纹伊蚊幼虫中诱导RNAi的效率。我们发现，V-ATP酶的表达被抑制高达90%，在转染后96小时在转染的C6/C36细胞。此外，各种RNAi表达AeDNV载体用于感染Ae.还测试了白纹伊蚊幼虫。我们发现，当Ae。重组AeDNV感染白纹伊蚊幼虫后，V-ATPase的表达较对照组下调近70%。此外，中位存活时间的生物测定表明，重组AeDNV引起更严重的致病作用比野生型病毒。这是第一个报告表明，重组病毒质粒和相应的重组AeDNV可以分别作为一个有效的体外和体内RNAi传递系统。
The Aedes aegypti densovirus (AeDNV) has potential as a delivery vector for foreign nucleic acids into mosquito cells. In this study, we investigated the ability of plasmids containing recombinant viral transducing genome to induce RNA interference (RNAi) effects in C6/C36 cells. We then evaluated the efficiency of a recombinant AeDNV vector to induce RNAi in Aedes albopictus larvae. We found that the expression of V-ATPase was inhibited by up to 90% at 96 h post-transfection in transfected C6/C36 cells. In addition, the bioinsecticidal activities of various RNAi-expressing AeDNV vectors used to infect Ae. albopictus larvae were also tested. We found that when Ae. albopictus larvae were infected with recombinant AeDNV, expression of V-ATPase was downregulated by nearly 70% compared to controls. Furthermore, the median survival time bioassays demonstrated that recombinant AeDNV caused more serious pathogenic effects than the wild type virus. This is the first report showing that recombinant virus plasmid and corresponding recombinant AeDNV can be used as an effective in vitro and in vivo RNAi delivery system, respectively.
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影响因子：
2.2
作者：
Huang, CG;Tsai, KH;Chen, WJ
通讯作者：
Chen, WJ
影响因子：
3.4
作者：
Barreau, C;Jousset, FX;Bergoin, M
通讯作者：
Bergoin, M
影响因子：
3.1
作者：
BARREAU, C;JOUSSET, FX;CORNET, M
通讯作者：
CORNET, M
影响因子：
2.1
作者：
AFANASIEV, BN;KOZLOV, YV;BEATY, BJ
通讯作者：
BEATY, BJ
DOI：
10.1073/pnas.93.23.13066
发表时间：
1996-11-12
影响因子：
11.1
作者：
Dimopoulos, G;Richman, A;Louis, C
通讯作者：
Louis, C