Removing Subcutaneous Microvessels Using Photo-Mediated Ultrasound Therapy.
Removing Subcutaneous Microvessels Using Photo-Mediated Ultrasound Therapy.
复制标题
使用光介导的超声疗法去除皮下微血管。
DOI：
10.1002/lsm.23260
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发表时间：
2020-12
影响因子：
2.4
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Wang M;Qin Y;Wang T;Orringer JS;Paulus YM;Yang X;Wang X
我们已经开发出一种新的抗血管技术，称为光介导的超声治疗（PUT），它利用纳秒持续时间的激光脉冲与超声脉冲同步，通过空化去除微血管。本研究的目的是探索PUT在去除皮下微血管方面的潜力。用峰值负压为0.45 MPa、频率为0.5 MHz、能量密度为0.056 J/cm ~ 2、波长为1064 nm的激光对2只新西兰白色兔耳廓血管进行超声治疗10 min。在治疗前和治疗后立即以及治疗后1小时、3天、2周和4周，通过商业激光散斑成像（LSI）系统测量治疗区域的血液灌注。在此时间段内跟踪来自4只兔耳的38条单独血管的灌注率以进行纵向评估。通过灌注率相对变化（RCPR）量化的治疗区域中血管的测量灌注率显示治疗后所有时间点的统计学显著降低（p<0.001）。治疗后即刻灌注平均减少50.79%，治疗后四周平均减少32.14%。治疗后即刻，灌注率迅速下降。在此之后，在治疗后3天内灌注率部分恢复，然后在3天至4周内灌注达到平台。这项研究表明，单次PUT治疗可以显著减少皮肤中32.14%的血液灌注长达4周。与光疗法相比，PUT具有激光能量密度低、无药物治疗等独特优势，有望成为治疗皮肤血管病变的新工具。
We have developed a novel anti-vascular technique, termed photo-mediated ultrasound therapy (PUT), which utilizes nanosecond duration laser pulses synchronized with ultrasound bursts to remove microvasculature through cavitation. The objective of the current study is to explore the potential of PUT in removing subcutaneous microvessels. The auricular blood vessels of two New Zealand white rabbits were treated by PUT with a peak negative ultrasound pressure of 0.45 MPa at 0.5 MHz, and a laser fluence of 0.056 J/cm2 at 1064 nm for 10 minutes. Blood perfusion in the treated area was measured by a commercial laser speckle imaging (LSI) system before and immediately after treatment, as well as at one hour, three days, two weeks, and four weeks post treatment. Perfusion rates of 38 individual vessels from 4 rabbit ears were tracked during this time period for longitudinal assessment. The measured perfusion rates of the vessels in the treated areas, as quantified by the relative change in perfusion rate (RCPR), showed a statistically significant decrease for all time points post treatment (p<0.001). The mean decrease in perfusion is 50.79% immediately after treatment and is 32.14% at four weeks post treatment. Immediately after treatment, the perfusion rate decreased rapidly. Following this, there was a partial recovery in perfusion rate up to 3 days post treatment, then followed by a plateau in the perfusion from 3 days to 4 weeks. This study demonstrated that a single PUT treatment could significantly reduce blood perfusion by 32.14% in the skin for up to 4 weeks. With unique advantages such as low laser fluence as compared with photothermolysis and agent-free treatment as compared with PDT, PUT holds potential to be developed into a new tool for the treatment of cutaneous vascular lesions.
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DOI：
10.1186/1546-0096-13-1
发表时间：
2015
期刊：
Pediatric rheumatology online journal
影响因子：
--
作者：
Chiewchengchol D;Murphy R;Edwards SW;Beresford MW
通讯作者：
Beresford MW
DOI：
10.1111/jdv.12021
发表时间：
2013-11-01
影响因子：
9.2
作者：
van Drooge, A. . M.;Bosveld, B.;Wolkerstorfer, A.
通讯作者：
Wolkerstorfer, A.
影响因子：
3.3
作者：
Chen, Defu;Ren, Jie;Gu, Ying
通讯作者：
Gu, Ying
影响因子：
3.4
作者：
Barton, JK;Izatt, JA;Welch, AJ
通讯作者：
Welch, AJ
影响因子：
3.5
作者：
Verkruysse, W;van Gemert, MJC;Nelson, JS
通讯作者：
Nelson, JS