Complex patterns of dopamine-related gene expression in the ventral tegmental area of male zebra finches relate to dyadic interactions with long-term female partners.
Complex patterns of dopamine-related gene expression in the ventral tegmental area of male zebra finches relate to dyadic interactions with long-term female partners.
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DOI：
10.1111/gbb.12619
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发表时间：
2020-02
期刊：
影响因子：
--
通讯作者：
Riters LV
中科院分区：
文献类型：
--
作者：
Alger SJ;Kelm-Nelson CA;Stevenson SA;Juang C;Gammie SC;Riters LV
从腹侧被盖区（VTA）到多个输出目标的多巴胺能投射与成对结合有关，但VTA在维持长期成对结合中的作用尚未得到很好的表征。许多神经调节剂之间的复杂相互作用改变了VTA的活性，这表明该区域基因表达模式的个体差异可以解释结合对长期社会互动的个体差异。为了验证这一假设，我们使用RNA-seq测量了雄性斑马雀中8000多个注释基因的表达。加权基因共表达网络分析发现一个包含大量多巴胺相关基因的基因模块，TH是该模块中联系最紧密的基因。该模块中的基因与男性的竞争行为以及女性伴侣产生的与结合相关的行为有关。无监督学习方法确定了两组在许多基因表达方面不同的雄性。富集分析显示，这些群体之间存在许多多巴胺相关基因和调节因子的差异，包括多巴胺受体、合成和降解酶、鸟类多巴胺转运蛋白以及一些GABA和谷氨酸相关基因。在两个雄性群体中，许多与VTA基因表达密切相关的结合行为是由雄性伴侣产生的，而不是雄性自己。总的来说，结果突出了VTA中的许多候选基因，这些基因可以在未来的研究中探索，并提出了VTA的分子/遗传组织可能受到社会伴侣和/或配对结合强度的强烈影响的可能性。
Dopaminergic projections from the ventral tegmental area (VTA) to multiple efferent targets are implicated in pair bonding, yet the role of the VTA in the maintenance of long-term pair bonds is not well characterized. Complex interactions between numerous neuromodulators modify activity in the VTA, suggesting that individual differences in patterns of gene expression in this region may explain individual differences in long-term social interactions in bonded pairs. To test this hypothesis we used RNA-seq to measure expression of over 8000 annotated genes in male zebra finches in established male-female pairs. Weighted gene co-expression network analysis identified a gene module that contained numerous dopamine-related genes with TH found to be the most connected gene of the module. Genes in this module related to male agonistic behaviors as well as bonding-related behaviors produced by female partners. Unsupervised learning approaches identified two groups of males that differed with respect to expression of numerous genes. Enrichment analyses revealed that many dopamine-related genes and modulators differed between these groups, including dopamine receptors, synthetic and degradative enzymes, the avian dopamine transporter, and several GABA- and glutamate-related genes. Many of the bonding-related behaviors closely associated with VTA gene expression in the two male groups were produced by the male’s partner, rather than the male himself. Collectively, results highlight numerous candidate genes in the VTA that can be explored in future studies and raise the possibility that the molecular/genetic organization of the VTA may be strongly shaped by a social partner and/or the strength of the pair bond.
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