Neuropeptidomics of the mosquito Aedes aegypti.
Neuropeptidomics of the mosquito Aedes aegypti.
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DOI：
10.1021/pr901187p
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发表时间：
2010-04-05
影响因子：
4.4
通讯作者：
Nachman, Ronald J.
中科院分区：
文献类型：
--
作者：
Predel, Reinhard;Neupert, Susanne;Garczynski, Stephen F.;Crim, Joe W.;Brown, Mark R.;Russell, William K.;Kahnt, Joerg;Russell, David H.;Nachman, Ronald J.
神经肽组数据收集的蚊子Ae。埃及伊蚊，其被认为是用于生理和内分泌研究的最易处理的蚊子物种。这些数据仅通过直接质谱分析获得，包括串联片段化，从单一标本中选择组织，这产生了推定生物活性神经肽的基本完整的核算;具有低丰度的截短神经肽不计为成熟肽。检测到CNS内CAPA前体的差异加工，并检测到AST-C和CAPA-PVK-2的差异翻译后加工（焦谷氨酸形成）。对于第一次在昆虫中，我们成功地在中肠组织的直接质谱分析，产生了一个全面的和即时的概述参与肠道内分泌系统的肽。较早被鉴定为Ae的最丰富的RFamide的头部肽。在CNS或中肠的任何部分均未检测到埃及伊蚊。本研究为蚊子内分泌学和神经生物学的进一步研究提供了一个框架。鉴于在其他医学上重要的蚊子中鉴定的神经肽前体的高度序列相似性，关于Ae.埃及伊蚊可能适用于这些蚊子。
Neuropeptidomic data were collected on the mosquito Ae. aegypti which is considered the most tractable mosquito species for physiological and endocrine studies. The data were solely obtained by direct mass spectrometric profiling, including tandem fragmentation, of selected tissues from single specimens which yielded a largely complete accounting of the putative bioactive neuropeptides; truncated neuropeptides with low abundance were not counted as mature peptides. Differential processing within the CNS was detected for the CAPA-precursor and differential post-translational processing (pyroglutamate formation) was detected for AST-C and CAPA-PVK-2. For the first time in insects, we succeeded in the direct mass spectrometric profiling of midgut tissue which yielded a comprehensive and immediate overview of the peptides involved in the endocrine system of the gut. Head peptides which were earlier identified as the most abundant RFamides of Ae. aegypti, were not detected in any part of the CNS or midgut. This study provides a framework for future investigations on mosquito endocrinology and neurobiology. Given the high sequence similarity of neuropeptide precursors identified in other medically important mosquitoes, conclusions regarding the peptidome of Ae. aegypti likely are applicable to these mosquitoes.
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影响因子：
4.8
作者：
Brown, MR;Graf, R;Lea, AO
通讯作者：
Lea, AO
DOI：
10.1016/j.bbrc.2005.07.038
发表时间：
2005-09-16
影响因子：
3.1
作者：
Cazzamali, G;Torp, M;Grimmelikhuijzen, CJP
通讯作者：
Grimmelikhuijzen, CJP
影响因子：
2.2
作者：
CLEMENTS, AN;POTTER, SA;SCALES, MDC
通讯作者：
SCALES, MDC
影响因子：
3.6
作者：
Hernández-Martínez, S;Li, YP;Noriega, FG
通讯作者：
Noriega, FG
DOI：
10.1093/jis/1.1.3
发表时间：
2001-01-01
期刊：
Journal of Insect Science (Tucson)
影响因子：
--
作者：
Brown, Mark R.;Cao, Chun
通讯作者：
Cao, Chun