Excitation-resolved fluorescence tomography with simplified spherical harmonics equations.
Excitation-resolved fluorescence tomography with simplified spherical harmonics equations.
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DOI：
10.1088/0031-9155/56/5/015
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发表时间：
2011-03-07
影响因子：
3.5
通讯作者：
Pöschinger T
中科院分区：
文献类型：
--
作者：
Klose AD;Pöschinger T
荧光断层扫描（FT）重建生物组织内的三维（3D）荧光报告探针分布。这些探针靶向生物功能分子，例如细胞表面受体或酶，并在用外部光源照射时发射荧光。在组织表面上检测荧光，并且基于简化球谐函数（SPN）方程的源重建算法计算组织内部的未知3D探针分布。虽然当前的FT方法需要在限定的波长范围内的多个外部源，但是所提出的FT方法仅使用具有可调谐波长选择的白色光源用于荧光刺激，并且为了3D断层摄影重建的目的进一步利用组织吸收的光谱依赖性。我们将通过实验数据证明所提出的高光谱激发分辨荧光层析成像方法的可行性。此外，我们将通过数字小鼠模型和合成测量数据来展示这种方法在理想和受控条件下的性能和局限性。此外，我们将解决有关荧光源重建所需的波长间隔量的问题。我们将探讨假设的空间均匀和不均匀的光学参数映射的荧光源重建的准确性的影响。最后，我们提出了一种光谱重新缩放方法，以克服所观察到的局限性，在重建准确的源分布在光学不均匀的组织时，假设只有均匀的光学特性映射的源重建过程。
Fluorescence tomography (FT) reconstructs the three-dimensional (3D) fluorescent reporter probe distribution inside biological tissue. These probes target molecules of biological function, e.g. cell surface receptors or enzymes, and emit fluorescence light upon illumination with an external light source. The fluorescence light is detected on the tissue surface and a source reconstruction algorithm based on the simplified spherical harmonics (SPN) equations calculates the unknown 3D probe distribution inside tissue. While current FT approaches require multiple external sources at a defined wavelength range, the proposed FT method uses only a white light source with tunable wavelength selection for fluorescence stimulation and further exploits the spectral dependence of tissue absorption for the purpose of 3D tomographic reconstruction. We will show the feasibility of the proposed hyperspectral excitation-resolved fluorescence tomography method with experimental data. In addition, we will demonstrate the performance and limitations of such a method under ideal and controlled conditions by means of a digital mouse model and synthetic measurement data. Moreover, we will address issues regarding the required amount of wavelength intervals for fluorescent source reconstruction. We will explore the impact of assumed spatially uniform and nonuniform optical parameter maps on the accuracy of the fluorescence source reconstruction. Last, we propose a spectral re-scaling method for overcoming the observed limitations in reconstructing accurate source distributions in optically non-uniform tissue when assuming only uniform optical property maps for the source reconstruction process.
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