Development and validation of a PCR-based functional marker system for the brown planthopper resistance gene Bph14 in rice.
Development and validation of a PCR-based functional marker system for the brown planthopper resistance gene Bph14 in rice.
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水稻褐飞虱抗性基因 Bph14 基于 PCR 的功能标记系统的开发和验证。
DOI：
10.1270/jsbbs.63.347
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发表时间：
2013-09
期刊：
影响因子：
2.4
通讯作者：
You A
中科院分区：
文献类型：
--
作者：
Zhou L;Chen Z;Lang X;Du B;Liu K;Yang G;Hu G;Li S;He G;You A
褐飞虱（BPH）是全球危害最严重的水稻害虫。抗虫品种是保护水稻免受褐飞虱危害的最有效和最环保的策略。从影响表型变异的基因序列中的多态性位点设计的功能标记（FM）用于标记辅助选择（MAS）时是非常有效的。Bph 14是迄今为止在水稻中克隆的第一个也是唯一的抗虫基因。与其非有效等位基因的序列相比，存在一些SNP差异。本研究采用等位基因特异性扩增（阿萨）的方法，设计了一个简单、共显性的Bph 14功能标记Bph 14 P/N。将Bph 14 P/N与两个特异性显性标记组合：一个标记Bph 14 P，靶向Bph 14的启动子区，扩增出566 bp的片段;另一个标记Bph 14 N，靶向Bph 14的LRR区，扩增出345 bp的片段。在两个水稻育种群体和一个中国水稻微型核心种质中验证了该功能标记系统的特异性和适用性。我们建议使用这种简单，低成本的标记系统在常规基因分型Bph 14在育种群体。
Brown planthopper (BPH) is the most damaging rice pest globally. Resistant varieties are the most effective and environmental strategy for protecting the rice crop from BPH. Functional markers (FMs) designed from polymorphic sites within gene sequences affecting phenotypic variation are highly efficient when used for marker assisted selection (MAS). Bph14 is the first and only cloned insect resistance gene so far in rice. Compared to the sequences of its non-effective alleles there are a number SNP differences. In this study, the method of allele-specific amplification (ASA) was adopted to design a simple, co-dominant, functional marker Bph14P/N for Bph14. Bph14P/N was combined with two specific dominant markers: one, named Bph14P, targets the promoter region of Bph14 and amplifies 566 bp fragments; and the other, Bph14N, targets the LRR region of bph14 and amplifies 345 bp fragments. Specificity and applicability of the functional marker system were verified in two breeding populations and a Chinese mini core collection of Oryza sativa. We recommend the use of this simple, low-cost marker system in routine genotyping for Bph14 in breeding populations.
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影响因子：
5.4
作者：
Rahman, Md Lutfor;Jiang, Wenzhu;Koh, Hee-Jong
通讯作者：
Koh, Hee-Jong
影响因子：
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作者：
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通讯作者：
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影响因子：
2
作者：
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影响因子：
2.2
作者：
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作者：
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