Response of cell-wall composition and RNA-seq transcriptome to methyl-jasmonate in Brachypodium distachyon callus.
Response of cell-wall composition and RNA-seq transcriptome to methyl-jasmonate in Brachypodium distachyon callus.
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DOI：
10.1007/s00425-018-2968-9
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发表时间：
2018-11
期刊：
影响因子：
4.3
通讯作者：
Mitchell RAC
中科院分区：
文献类型：
--
作者：
Hyde LS;Pellny TK;Freeman J;Michaelson LV;Simister R;McQueen-Mason SJ;Mitchell RAC
茉莉酸甲酯诱导大幅度增加对香豆酸连接到阿拉伯木聚糖在短柄草属和丰富的GT 61和BAHD家族转录与合成的作用，这种联系。茉莉酸（JA）信号是植物许多胁迫反应所必需的，诱导转录组的巨大变化，包括与木质化相关的转录本的上调。然而，知之甚少的响应JA的草细胞壁和阿拉伯木聚糖（AX）的合成和酰化的阿魏酸（FA）和对香豆酸（pCA）的单子叶植物的具体功能。在这里，我们表明，茉莉酸甲酯（MeJA）诱导适度增加FA单体，增加> 50%的FA二聚体，并增加五至六倍的pCA酯连接到短柄草愈伤组织的细胞壁。直接测量由pCA酰化的阿拉伯糖（Araf-pCA）表明，细胞壁pCA的大部分或全部增加是由于pCA酯连接到AX。对愈伤组织响应的RNA-seq转录组的分析表明，这些细胞壁变化伴随着涉及AX装饰和酰化的GT 61和BAHD基因家族成员的上调;两个BAHD旁系同源物在暴露于MeJA 24小时后是上调最多的细胞壁基因（7倍和5倍）。在水稻幼苗的RiceXPro公开表达数据集中，orthopedium BAHD和GT 61转录物对JA的反应相似，表明它们对短柄草属或愈伤组织不是特异性的。因此，AX-pCA对MeJA的大响应可能表明这种联系在草的初级细胞壁对JA信号的响应中起重要作用。本文的在线版本（10.1007/s 00425 -018-2968-9）包含补充材料，可供授权用户使用。
Methyl-jasmonate induces large increases in p-coumarate linked to arabinoxylan in Brachypodium and in abundance of GT61 and BAHD family transcripts consistent with a role in synthesis of this linkage. Jasmonic acid (JA) signalling is required for many stress responses in plants, inducing large changes in the transcriptome, including up-regulation of transcripts associated with lignification. However, less is known about the response to JA of grass cell walls and the monocot-specific features of arabinoxylan (AX) synthesis and acylation by ferulic acid (FA) and para-coumaric acid (pCA). Here, we show that methyl-jasmonate (MeJA) induces moderate increases in FA monomer, > 50% increases in FA dimers, and five–sixfold increases in pCA ester-linked to cell walls in Brachypodium callus. Direct measurement of arabinose acylated by pCA (Araf-pCA) indicated that most or all the increase in cell-wall pCA was due to pCA ester-linked to AX. Analysis of the RNA-seq transcriptome of the callus response showed that these cell-wall changes were accompanied by up-regulation of members of the GT61 and BAHD gene families implicated in AX decoration and acylation; two BAHD paralogues were among the most up-regulated cell-wall genes (seven and fivefold) after 24 h exposure to MeJA. Similar responses to JA of orthologous BAHD and GT61 transcripts are present in the RiceXPro public expression data set for rice seedlings, showing that they are not specific to Brachypodium or to callus. The large response of AX-pCA to MeJA may, therefore, indicate an important role for this linkage in response of primary cell walls of grasses to JA signalling. The online version of this article (10.1007/s00425-018-2968-9) contains supplementary material, which is available to authorized users.
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DOI：
10.1111/tpj.12420
发表时间：
2014-03
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Petrik DL;Karlen SD;Cass CL;Padmakshan D;Lu F;Liu S;Le Bris P;Antelme S;Santoro N;Wilkerson CG;Sibout R;Lapierre C;Ralph J;Sedbrook JC
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DOI：
10.1111/nph.14970
发表时间：
2018-04
期刊：
The New phytologist
影响因子：
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Molinari HBC
影响因子：
4.3
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Dubcovsky J
DOI：
10.1073/pnas.1202079109
发表时间：
2012-10-16
影响因子：
11.1
作者：
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通讯作者：
Ronald, Pamela C.
DOI：
10.1073/pnas.92.10.4114
发表时间：
1995-05-09
影响因子：
11.1
作者：
CREELMAN, RA;MULLET, JE
通讯作者：
MULLET, JE