Singularity response reveals entrainment properties in mammalian circadian clock.
Singularity response reveals entrainment properties in mammalian circadian clock.
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DOI：
10.1038/s41467-023-38392-x
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发表时间：
2023-05-17
影响因子：
16.6
通讯作者：
Hirano, Arisa
中科院分区：
文献类型：
--
作者：
Masuda, Kosaku;Kon, Naohiro;Iizuka, Kosuke;Fukada, Yoshitaka;Sakurai, Takeshi;Hirano, Arisa
夹带作用的特征是相响应曲线(PRCs)，它提供了对每个昼夜节律阶段的扰动的响应的汇总。哺乳动物生物钟的同步是通过接收来自内部和外部时间提示的各种输入来完成的。需要对每个组织中不同刺激的原癌细胞进行全面的比较。在这里，我们证明了哺乳动物细胞中的原癌细胞可以使用最近发展的基于奇异性响应(SR)的估计方法来表征，奇异性响应(SR)代表去同步细胞时钟的响应。我们证实，在几个细胞系中，可以通过单次SR测量和对不同刺激的量化反应属性来重建PRCs。随机共振分析表明，重置后的相位和幅度在刺激之间是可区分的。组织切片培养中的SRS揭示了组织特有的夹带特性。这些结果表明，SRS可以用来揭示多尺度哺乳动物时钟中不同刺激的携带机制。目前评估昼夜节律生物参数的方法可能是劳动密集型的。在这里，作者建立了一种通过简单的实验和数学建模来估计昼夜节律特征的方法，揭示了哺乳动物昼夜节律对不同刺激的反应。
Entrainment is characterized by phase response curves (PRCs), which provide a summary of responses to perturbations at each circadian phase. The synchronization of mammalian circadian clocks is accomplished through the receipt of a variety of inputs from both internal and external time cues. A comprehensive comparison of PRCs for various stimuli in each tissue is required. Herein, we demonstrate that PRCs in mammalian cells can be characterized using a recently developed estimation method based on singularity response (SR), which represents the response of desynchronized cellular clocks. We confirmed that PRCs can be reconstructed using single SR measurements and quantified response properties for various stimuli in several cell lines. SR analysis reveals that the phase and amplitude after resetting are distinguishable among stimuli. SRs in tissue slice cultures reveal tissue-specific entrainment properties. These results demonstrate that SRs can be employed to unveil entrainment mechanisms with diverse stimuli in multiscale mammalian clocks. Current methods to assess circadian biological parameters can be labor intensive. Here, the authors establish a method for estimating circadian entrainment characteristics using simple experiments and mathematical modeling, revealing the responsiveness of circadian rhythms to diverse stimuli in the mammalian circadian clock.
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影响因子：
21.3
作者：
Ukai, Hideki;Kobayashi, Tetsuya J.;Ueda, Hiroki R.
通讯作者：
Ueda, Hiroki R.
昼夜节律对衰老和寿命的重要性。
影响因子：
16.6
作者：
Acosta-Rodríguez VA;Rijo-Ferreira F;Green CB;Takahashi JS
通讯作者：
Takahashi JS
影响因子：
11.2
作者：
Shafi AA;Knudsen KE
通讯作者：
Knudsen KE
影响因子：
29
作者：
Hatori M;Vollmers C;Zarrinpar A;DiTacchio L;Bushong EA;Gill S;Leblanc M;Chaix A;Joens M;Fitzpatrick JA;Ellisman MH;Panda S
通讯作者：
Panda S
影响因子：
56.9
作者：
Balsalobre, A;Brown, SA;Schibler, U
通讯作者：
Schibler, U