iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of Euphorbia kansui L.
iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of Euphorbia kansui L.
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基于 iTRAQ 的蛋白质组学分析甘水大戟乳汁中自噬介导的 MeJA 反应。
DOI：
10.3390/ijms20153770
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发表时间：
2019-08
影响因子：
5.6
通讯作者：
Xia Cai
中科院分区：
文献类型：
--
作者：
Xiaoai Fang;Xiangyu Yao;Yue Zhang;Zheni Tian;Meng Wang;Peng Li;Xia Cai
自噬是一种明确的分解代谢机制，胞质物质被吞噬到一种称为自噬小体的结构中。茉莉酸甲酯(MeJA)是一种植物激素，它介导多种发育过程和防御反应，诱导多种代谢产物。在植物中，对自噬介导的对MeJA的反应知之甚少。在这项研究中，我们使用高通量比较蛋白质组学来鉴定乳管中的乳胶蛋白。利用等压相对和绝对定量(ITRAQ)MS/MS蛋白质组学方法，鉴定了甘遂大戟处理组和对照组的298个蛋白质。值得注意的是，鉴定出29个显著差异表达的蛋白质，并验证了它们与自噬和ROS途径的关系。这些蛋白质包括：α-L岩藻糖苷酶、β-半乳糖苷酶、半胱氨酸蛋白酶和铜/锌超氧化物歧化酶。对所选基因的实时定量聚合酶链式反应分析证实，MeJA可能会增强一些与自噬相关的基因的表达。自噬形成的两个重要蛋白质ATG8和ATG18a的Western blotting和免疫荧光结果也表明，MeJA在蛋白质水平上可以促进自噬。用电子显微镜观察到MeJA处理后自噬小体的增加。这些结果表明，MeJA可能促进甘遂乳管的自噬，推测MeJA可能通过两种途径介导自噬：ROS的增加诱导ATG8的积累，进而形成自噬小体；MeJA促进ATG18的积累，进而形成自噬小体。综上所述，我们的结果为理解自噬和MeJA治疗之间的机制提供了几个新的见解。但具体作用机制有待于今后进一步研究。
Autophagy is a well-defined catabolic mechanism whereby cytoplasmic materials are engulfed into a structure termed the autophagosome. Methyl jasmonate (MeJA), a plant hormone, mediates diverse developmental process and defense responses which induce a variety of metabolites. In plants, little is known about autophagy-mediated responses against MeJA. In this study, we used high-throughput comparative proteomics to identify proteins of latex in the laticifers. The isobaric tags for relative and absolute quantification (iTRAQ) MS/MS proteomics were performed, and 298 proteins among MeJA treated groups and the control group of Euphorbia kansui were identified. It is interesting to note that 29 significant differentially expressed proteins were identified and their associations with autophagy and ROS pathway were verified for several selected proteins as follows: α-L-fucosidase, β-galactosidase, cysteine proteinase, and Cu/Zn superoxide dismutase. Quantitative real-time PCR analysis of the selected genes confirmed the fact that MeJA might enhance the expression of some genes related to autophagy. The western blotting and immunofluorescence results of ATG8 and ATG18a which are two important proteins for the formation of autophagosomes also demonstrated that MeJA could promote autophagy at the protein level. Using the electron microscope, we observed an increase in autophagosomes after MeJA treatment. These results indicated that MeJA might promote autophagy in E. kansui laticifers; and it was speculated that MeJA mediated autophagy through two possible ways: the increase of ROS induces ATG8 accumulation and then aotophagosome formation, and MeJA promotes ATG18 accumulation and then autophagosome formation. Taken together, our results provide several novel insights for understanding the mechanism between autophagy and MeJA treatment. However, the specific mechanism remains to be further studied in the future.
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影响因子：
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作者：
Xiaoai Fang;Yue Zhang;Meng Wang;Peng Li;Qing Zhang;Jingjing Si;Bofei Wei;Yan Miao;Lanting Tian;Xia Cai
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期刊：
Journal of Protein Chemistry
影响因子：
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DOI：
10.1134/s0006297916040052
发表时间：
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期刊：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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