Lysosome and proteasome pathways are distributed in laticifers of Euphorbia helioscopia L.
Lysosome and proteasome pathways are distributed in laticifers of Euphorbia helioscopia L.
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溶酶体和蛋白酶体途径分布于大戟的乳汁中。
DOI：
10.1111/ppl.12869
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发表时间：
2019-08
影响因子：
6.4
通讯作者：
Xia Cai
中科院分区：
文献类型：
--
作者：
Xiaoai Fang;Yue Zhang;Meng Wang;Peng Li;Qing Zhang;Jingjing Si;Bofei Wei;Yan Miao;Lanting Tian;Xia Cai
目前，溶酶体途径（LP）和蛋白酶体途径（PP）被认为是真核细胞的主要清除系统。乳管是一种分泌组织，在发育过程中降解一些细胞质。本研究对泽漆无节乳管中脂蛋白和聚丙烯蛋白的分布进行了研究。电镜观察发现，发育后期乳管中质体、线粒体和细胞质均发生降解，并出现大量的由双细胞体形成的小泡。因此，在大肠杆菌中检测到LP和PP中的一些关键蛋白。使用同量异位素标签进行相对和绝对定量（iTRAQ）蛋白质组学的日光灯下乳胶。进一步的免疫组织化学分析表明，属于LP的网格蛋白重链（CHC）和泛素介导的蛋白酶体降解随着乳管的发育逐渐增加。免疫电镜观察发现，LP的半胱氨酸蛋白酶、CHC和AP-2复合物β 1亚基主要分布在由双胞体衍生的囊泡中，我们称之为溶酶体样囊泡。泛素广泛分布在胞质溶胶中，蛋白酶体活性显着降低时，各种浓度的抑制剂MG 132被添加到胶乳总蛋白。我们假设LP和PP在E.推测LP和PP可能参与了E.乳管日光花
At present, the lysosome pathway (LP) and proteasome pathway (PP) are known as major clearance systems in eukaryotic cells. The laticifer, a secretory tissue, degrades some cytoplasm during development. In this study, we investigated the distribution of LP and PP in non-articulated laticifers of Euphorbia helioscopia L. Electron microscopy revealed that, plastids, mitochondria and some cyotsol were degraded in the late development laticifers, where there were numerous vesicles originated from dicytosomes. Accordingly, some key proteins in LP and PP were detected in E. helioscopia latex using isobaric tags for relative and absolute quantitation (iTRAQ) proteomics. Further immunohistochemistry analysis revealed that the clathrin heavy chain (CHC) belonging to LP and the ubiquitin-mediated proteasome degradation increases gradually as the laticifer develops. Immuno-electron microscopy revealed that the cysteine protease, CHC and AP-2 complex subunit beta-1 belonging to LP were mainly distributed in vesicles deriving from dicytosomes, which we called lysosome-like vesicles. Ubiquitin was widely distributed in the cytosol, and proteasome activity was significantly reduced when various concentrations of the inhibitor MG132 were added to the latex total protein. We hypothesize that LP and PP are distributed in E. helioscopia laticifers; and it was speculated that LP and PP might be involved in the degradation of organelles and some cytoplasmic matrix in E. helioscopia laticifers.
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影响因子：
3.8
作者：
Nawrot, Robert;Kalinowski, Andrzej;Gozdzicka-Jozefiak, Anna
通讯作者：
Gozdzicka-Jozefiak, Anna
影响因子：
3.9
作者：
S. Rawdkuen;P. Chaiwut;P. Pintathong;S. Benjakul
通讯作者：
S. Rawdkuen;P. Chaiwut;P. Pintathong;S. Benjakul
影响因子：
4.2
作者：
J. Inamdar;V. Murugan;R. Subramanian
通讯作者：
J. Inamdar;V. Murugan;R. Subramanian
DOI：
10.1023/a:1023059525861
发表时间：
2003
期刊：
Journal of Protein Chemistry
影响因子：
--
作者：
Sandra Vairo Cavalli;M. Arribére;A. Cortadi;N. Caffini;N. Priolo
通讯作者：
Sandra Vairo Cavalli;M. Arribére;A. Cortadi;N. Caffini;N. Priolo
影响因子：
11.4
作者：
BACHMAIR, A;BECKER, F;SCHELL, J
通讯作者：
SCHELL, J