Characterization of Alternative Splicing (AS) Events during Chicken (Gallus gallus) Male Germ-Line Stem Cell Differentiation with Single-Cell RNA-seq.
Characterization of Alternative Splicing (AS) Events during Chicken (Gallus gallus) Male Germ-Line Stem Cell Differentiation with Single-Cell RNA-seq.
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DOI：
10.3390/ani11051469
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发表时间：
2021-05-20
期刊：
影响因子：
--
通讯作者：
Li B
中科院分区：
文献类型：
--
作者：
Sun C;Jin K;Zuo Q;Sun H;Song J;Zhang Y;Chen G;Li B
研究表明，选择性剪接（AS）已被用于各种各样的生命过程。然而，关于AS在生殖细胞发育过程中的研究很少。本研究通过对鸡胚胎干细胞（ESCs）、性腺PGCs（gPGCs）和精原细胞干细胞（SSC）的RNA-seq数据分析，初步探讨了鸡生殖细胞形成过程中可变剪切事件的变化，以及生殖细胞发育过程中几个关键阶段特异性基因的关键AS模式。本研究结果为进一步分析参与生殖细胞形成的关键基因的调控机制奠定了理论基础。选择性剪接（alternative splicing，AS）是生殖细胞分化等发育过程中普遍存在的一种共转录和转录后调控机制。对生殖细胞分化的深入了解将有助于我们为鸟类生殖、干细胞生物学和人类消费药物的发展开辟新的途径。在这里，基于单细胞RNA-seq，我们表征了多种鸡雄性生殖细胞中的全基因组AS事件：胚胎干细胞（ESC），性腺原始生殖细胞（gPGC）和精原细胞干细胞（SSC）。在ESC中检测到来自15，338个基因的总共38，494个AS事件，在gPGC和SSC中分别观察到来自14，783个基因的总共48，955个事件和来自15，089个基因的49，900个事件。此外，AS事件的这种分布表明ESC、gPGC和SSC的不同剪接特征。最后，在胚胎干细胞、gPGC和干细胞中传播的AS事件中鉴定了几个关键的阶段特异性基因，例如NANOG、POU 5 F3、LIN 28 B、BMP 4、STRA 8和LHX 9。通过qRT-PCR验证RNA-seq数据的基因表达结果。总之，我们提供了一个全面的图谱的全基因组规模的AS事件景观在雄性鸡生殖系细胞，并首次提出了它的分布。这项研究可能有一天会改善男性不育症患者的治疗选择。
Studies have shown that alternative splicing (AS) has been utilized in a wide variety of life processes. However, there are very few studies on AS during germ cell development. In this study, we preliminarily investigated the variation of variable shear events during the formation of chicken germ cells through the RNA-seq data analysis of embryonic stem cells (ESCs), gonad PGCs (gPGCs), and spermatogonia stem cells (SSCs), and the critical AS mode for several crucial stage-specific genes, which were identified during germ cell development. The results of this study lay a theoretical foundation for further analysis of the regulation mechanism of key genes involved in germ cell formation. Alternative splicing (AS) is a ubiquitous, co-transcriptional, and post-transcriptional regulation mechanism during certain developmental processes, such as germ cell differentiation. A thorough understanding of germ cell differentiation will help us to open new avenues for avian reproduction, stem cell biology, and advances in medicines for human consumption. Here, based on single-cell RNA-seq, we characterized genome-wide AS events in manifold chicken male germ cells: embryonic stem cells (ESCs), gonad primordial germ cells (gPGCs), and spermatogonia stem cells (SSCs). A total of 38,494 AS events from 15,338 genes were detected in ESCs, with a total of 48,955 events from 14,783 genes and 49,900 events from 15,089 genes observed in gPGCs and SSCs, respectively. Moreover, this distribution of AS events suggests the diverse splicing feature of ESCs, gPGCs, and SSCs. Finally, several crucial stage-specific genes, such as NANOG, POU5F3, LIN28B, BMP4, STRA8, and LHX9, were identified in AS events that were transmitted in ESCs, gPGCs, and SSCs. The gene expression results of the RNA-seq data were validated by qRT-PCR. In summary, we provided a comprehensive atlas of the genome-wide scale of the AS event landscape in male chicken germ-line cells and presented its distribution for the first time. This research may someday improve treatment options for men suffering from male infertility.
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影响因子：
8.8
作者：
Hannigan MM;Zagore LL;Licatalosi DD
通讯作者：
Licatalosi DD
DOI：
10.1038/nrm.2017.27
发表时间：
2017-07
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Baralle FE;Giudice J
通讯作者：
Giudice J
DOI：
10.1111/j.1749-6632.1980.tb15971.x
发表时间：
1980-01-01
影响因子：
5.2
作者：
Jaffe, L.F.
通讯作者：
Jaffe, L.F.
影响因子：
10.5
作者：
Lawson, KA;Dunn, NR;Hogan, BLM
通讯作者：
Hogan, BLM
鸡模型中生长的基因组学。
影响因子：
4.4
作者：
Johnsson M;Henriksen R;Höglund A;Fogelholm J;Jensen P;Wright D
通讯作者：
Wright D