Coinfection and Genetic Characterization of Porcine Astrovirus in Diarrheic Piglets in China From 2015 to 2018
Coinfection and Genetic Characterization of Porcine Astrovirus in Diarrheic Piglets in China From 2015 to 2018
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2015-2018年我国腹泻仔猪猪星状病毒混合感染情况及遗传特征
DOI：
10.3389/fvets.2020.00462
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发表时间：
2020-08
影响因子：
3.2
通讯作者：
Dongbo Sun
中科院分区：
文献类型：
--
作者：
Mingjun Su;Shanshan Qi;Dan Yang;Donghua Guo;Baishuang Yin;Dongbo Sun
猪星状病毒（PAstV）在全球广泛分布，并以至少五种不同的基因型存在。 PAstV是最近被确定为仔猪腹泻的重要病原菌，在我国广泛分布。然而，很少有研究调查中国腹泻仔猪中 PAstV 的共感染和遗传特征。本研究在2015年至2018年中国543份腹泻样本中鉴定出89份PAstV阳性样本，其中75.28%（67/89）同时感染3至5种不同的猪病原菌，而无一例仅PAstV阳性。在 543 个腹泻样本中，统计分析显示 PAstV 引起的腹泻可能与 PEV (p < 0.01) 和 GARV (p < 0.01) 的双重感染有关。系统发育分析显示，27株已鉴定的PAstV毒株分属于3个不同的基因型，其中PAstV-2（81.48%，22/27）在我国腹泻仔猪中占优势，其次是PAstV-4（11.11%，3/27）和PAasV-5（7.41%，2/27）。序列分析显示，本研究中鉴定的 27 个 RdRp 基因的核苷酸同源性为 53.8-99.5%。此外，与其他已知的PAstV-4毒株相比，PAstV-4毒株JL/MHK/2018/0115的RdRp基因具有独特的三个核苷酸插入片段(GAA)。此外，尽管PAstV-2在中国分布最广泛，但PAstV的基因型在不同地理位置之间存在差异。这些数据表明，PAstV与其他猪病原体的混合感染很常见，并且在中国腹泻仔猪中PAstV存在遗传多样性。
Porcine astrovirus (PAstV) is broadly distributed globally and exists as at least five distinct genotypes. PAstV, which was recently identified as an important pathogen of diarrhea in piglets, is widely distributed in China. However, few studies have investigated the coinfection and genetic characterization of PAstV in diarrheic piglets in China. In this study, 89 PAstV-positive samples were identified in 543 diarrhea samples in China from 2015 to 2018, of which 75.28% (67/89) were coinfected with three to five different porcine pathogens, while none were positive for PAstV only. Among the 543 diarrhea samples, statistical analysis showed that PAstV-induced diarrhea was potentially associated with coinfection of PEV (p < 0.01) and GARV (p < 0.01). Phylogenetic analysis showed that the 27 identified PAstV strains belong to three different genotypes and that PAstV-2 (81.48%, 22/27) was predominant in diarrheic piglets in China, followed by PAstV-4 (11.11%, 3/27) and PAasV-5 (7.41%, 2/27). Sequence analysis revealed that the 27 RdRp genes identified in this study had nucleotide homologies of 53.8–99.5%. In addition, the RdRp gene of PAstV-4 strain JL/MHK/2018/0115 harbored a unique insert of three nucleotides (GAA) as compared with other known PAstV-4 strains. Furthermore, the genotypes of PAstV varied among different geographical locations, although PAstV-2 was the most widely distributed in China. These data demonstrate that PAstV coinfection with other porcine pathogens was common and there was genetic diversity of PAstV in diarrheic piglets in China.
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作者：
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