Visible light reduces C. elegans longevity.
Visible light reduces C. elegans longevity.
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DOI：
10.1038/s41467-018-02934-5
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发表时间：
2018-03-02
影响因子：
16.6
通讯作者：
Dillin A
中科院分区：
文献类型：
--
作者：
De Magalhaes Filho CD;Henriquez B;Seah NE;Evans RM;Lapierre LR;Dillin A
透明的线虫——秀丽隐杆线虫能够感知紫外线和蓝紫光，进而改变自身行为。由于高剂量的紫外线和蓝紫光并非实验室环境之外的常见因素，我们不禁思考，低强度可见光在秀丽隐杆线虫的生理机能和寿命方面是否发挥着某种作用。在此，我们发现秀丽隐杆线虫的寿命与线虫暴露于可见光下的时长呈负相关。虽然昼夜节律调控、lite - 1基因和tax - 2基因与寿命缩短并无关联，但我们证实可见光会引发光氧化应激以及普遍的未折叠蛋白反应，从而缩短寿命。最后，我们发现长寿突变体对光应激的耐受性更强，经抗氧化剂药物处理的野生型线虫同样如此。这项研究表明，透明线虫对可见光辐射敏感，同时也凸显了在实验室条件下进行寿命研究时，有必要规范控制光照这一未被识别的偏差效应的方法。
已知线虫秀丽隐杆线虫会对紫外线做出行为改变。本文作者指出，可见光会在秀丽隐杆线虫体内引发光氧化应激以及更广泛的应激反应，这表明在常规实验室操作过程中的光照可能会影响寿命实验的结果。
The transparent nematode Caenorhabditis elegans can sense UV and blue-violet light to alter behavior. Because high-dose UV and blue-violet light are not a common feature outside of the laboratory setting, we asked what role, if any, could low-intensity visible light play in C. elegans physiology and longevity. Here, we show that C. elegans lifespan is inversely correlated to the time worms were exposed to visible light. While circadian control, lite-1 and tax-2 do not contribute to the lifespan reduction, we demonstrate that visible light creates photooxidative stress along with a general unfolded-protein response that decreases the lifespan. Finally, we find that long-lived mutants are more resistant to light stress, as well as wild-type worms supplemented pharmacologically with antioxidants. This study reveals that transparent nematodes are sensitive to visible light radiation and highlights the need to standardize methods for controlling the unrecognized biased effect of light during lifespan studies in laboratory conditions. The nematode C. elegans is known to alter its behavior in response to UV light. Here, the authors show that visible light triggers photo-oxidative stress and a wider stress response in C. elegans, suggesting that light exposure during routine laboratory handling may influence the outcome of lifespan experiments.
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影响因子：
16.2
作者：
Bhatla, Nikhil;Horvitz, H. Robert
通讯作者：
Horvitz, H. Robert
影响因子：
64.5
作者：
Durieux J;Wolff S;Dillin A
通讯作者：
Dillin A
DOI：
10.1016/s0165-7992(98)90003-3
发表时间：
1988-06-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
HARTMAN, PS;SIMPSON, VJ;MITCHELL, D
通讯作者：
MITCHELL, D
影响因子：
4.8
作者：
Honda, Y;Honda, S
通讯作者：
Honda, S
DOI：
10.1093/gerona/55.5.b215
发表时间：
2000-05-01
影响因子：
5.1
作者：
Gems, D;Riddle, DL
通讯作者：
Riddle, DL