Light and hydrogen peroxide inhibit C. elegans Feeding through gustatory receptor orthologs and pharyngeal neurons.
Light and hydrogen peroxide inhibit C. elegans Feeding through gustatory receptor orthologs and pharyngeal neurons.
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DOI：
10.1016/j.neuron.2014.12.061
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发表时间：
2015-02-18
期刊：
影响因子：
16.2
通讯作者：
Horvitz, H. Robert
中科院分区：
文献类型：
--
作者：
Bhatla, Nikhil;Horvitz, H. Robert
虽然对五种主要味道（甜、咸、酸、苦和咸）的味觉感知已经被广泛研究，但检测非规范味觉刺激的途径仍然相对未被探索。特别是，虽然活性氧对生物系统造成广泛的损害，但在任何生物体中都没有发现防止摄入这种物质的味觉机制。我们观察到光抑制秀丽隐杆线虫的摄食，并利用光作为揭示味觉的分子和神经机制的工具。光可以产生过氧化氢，我们发现过氧化氢同样会抑制进食。味觉受体家族成员LITE-1和gur3是抑制光和过氧化氢摄食所必需的。I2咽部神经元对光和过氧化氢的反应增加钙，这些反应需要gur3和保守的抗氧化酶过氧化物酶PRDX-2。我们的结果证明了一种味觉机制，介导检测和阻止摄取非规范的味觉刺激，过氧化氢。
While gustatory sensing of the five primary flavors (sweet, salty, sour, bitter, and savory) has been extensively studied, pathways that detect non-canonical taste stimuli remain relatively unexplored. In particular, while reactive oxygen species cause generalized damage to biological systems, no gustatory mechanism to prevent ingestion of such material has been identified in any organism. We observed that light inhibits C. elegans feeding and used light as a tool to uncover molecular and neural mechanisms for gustation. Light can generate hydrogen peroxide, and we discovered that hydrogen peroxide similarly inhibits feeding. The gustatory receptor family members LITE-1 and GUR-3 are required for the inhibition of feeding by light and hydrogen peroxide. The I2 pharyngeal neurons increase calcium in response to light and hydrogen peroxide, and these responses require GUR-3 and a conserved antioxidant enzyme peroxiredoxin PRDX-2. Our results demonstrate a gustatory mechanism that mediates the detection and blocks ingestion of a non-canonical taste stimulus, hydrogen peroxide.
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