Integron Digestive Carriage in Human and Cattle: A "One Health" Cultivation-Independent Approach.
Integron Digestive Carriage in Human and Cattle: A "One Health" Cultivation-Independent Approach.
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DOI：
10.3389/fmicb.2017.01891
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发表时间：
2017
影响因子：
5.2
通讯作者：
Ploy MC
中科院分区：
文献类型：
--
作者：
Chainier D;Barraud O;Masson G;Couve-Deacon E;François B;Couquet CY;Ploy MC
目的：抗生素耐药性（AMR）的传播是一个全球性问题，需要采取“一个健康”的方法，促进人类和动物健康的整合。除了常用于AMR监测的培养依赖性技术外，非培养依赖性方法还可以对AMR遗传决定因素的多样性和储存库提供额外的见解。整合子是一种分子标记物，可以提供AMR传播的全面和可靠的估计。在这项研究中，考虑到“一个健康”的方法，我们分析了整合子消化运输从粪便中的人和牛生活在同一地区，并暴露于不同的抗生素选择压力。方法：分析了三组人类[普通人群（GP）和重症监护室患者（ICU）]和牛（BOV）粪便样本。使用多重qPCR从粪便提取的总DNA和富集步骤后培养获得的革兰氏阴性菌中检测到三种主要类型的整合子。结果如下：与培养无关的方法，整合子携带GP，ICU，和BOV分别为43.8，52.7，和65.6%，百分比是至少两倍以上的培养依赖性的方法获得的。1类整合子是最普遍的; 2类整合子似乎更相关的牛比人类;没有检测到3类整合子。根据抗生素消耗量，无论何种方法，GP和ICU人群之间的整合子携带没有显著差异。结论：培养独立的方法构成了一种补充的探索性方法，以研究整合子消化运输的人和牛，特别是在受试者接受抗生素治疗。肠道中整合子的高频率携带具有临床意义，整合子能够在抗生素选择压力下容易地获得和交换抗性基因，从而导致抗性细菌的传播。
Objectives: Dissemination of antimicrobial resistance (AMR) is a global issue that requires the adoption of a “One-Health” approach promoting integration of human and animal health. Besides culture-dependent techniques frequently used for AMR surveillance, cultivation-independent methods can give additional insights into the diversity and reservoir of AMR genetic determinants. Integrons are molecular markers that can provide overall and reliable estimation of AMR dissemination. In this study, considering the “One-Health” approach, we have analyzed the integron digestive carriage from stools of humans and cattle living in a same area and exposed to different antibiotic selection pressures. Methods: Three collections of human [general population (GP) and intensive care unit patients (ICUs)] and bovine (BOV) stool samples were analyzed. The three main classes of integrons were detected using a multiplex qPCR both from total DNA extracted from stools, and from Gram-negative bacteria obtained by culture after an enrichment step. Results: With the cultivation-independent approach, integron carriage was 43.8, 52.7, and 65.6% for GP, ICU, and BOV respectively, percentages being at least twofold higher to those obtained with the cultivation-dependent approach. Class 1 integrons were the most prevalent; class 2 integrons seemed more associated to cattle than to humans; no class 3 integron was detected. The integron carriage was not significantly different between GP and ICU populations according to the antibiotic consumption, whatever the approach. Conclusion: The cultivation-independent approach constitutes a complementary exploratory method to investigate the integron digestive carriage of humans and bovines, notably within subjects under antibiotic treatment. The high frequency of carriage of integrons in the gut is of clinical significance, integrons being able to easily acquire and exchange resistant genes under antibiotic selective pressure and so leading to the dissemination of resistant bacteria.
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影响因子：
5.2
作者：
Barraud, O.;Baclet, M. C.;Ploy, M. C.
通讯作者：
Ploy, M. C.
影响因子：
4
作者：
Barlow, R. S.;Fegan, N.;Gobius, K. S.
通讯作者：
Gobius, K. S.
DOI：
10.1016/j.ijantimicag.2014.06.008
发表时间：
2014-10-01
影响因子：
10.8
作者：
Barraud, Olivier;Francois, Bruno;Ploy, Marie-Cecile
通讯作者：
Ploy, Marie-Cecile
影响因子：
4.9
作者：
Copur-Cicek, Aysegul;Ozgumus, Osman Birol;Sandalli, Cemal
通讯作者：
Sandalli, Cemal
DOI：
10.1073/pnas.0306466101
发表时间：
2004-05-04
影响因子：
11.1
作者：
Nandi, S;Maurer, JJ;Summers, AO
通讯作者：
Summers, AO