Overexpression of ORCA3 and G10H in Catharanthus roseus plants regulated alkaloid biosynthesis and metabolism revealed by NMR-metabolomics.
Overexpression of ORCA3 and G10H in Catharanthus roseus plants regulated alkaloid biosynthesis and metabolism revealed by NMR-metabolomics.
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NMR 代谢组学揭示长春花植物中 ORCA3 和 G10H 的过度表达调节生物碱生物合成和代谢。
DOI：
10.1371/journal.pone.0043038
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Tang K
中科院分区：
文献类型：
--
作者：
Pan Q;Wang Q;Yuan F;Xing S;Zhao J;Choi YH;Verpoorte R;Tian Y;Wang G;Tang K
为了提高长春花中抗癌二聚吲哚生物碱的产量，人们致力于培养细胞系、毛状根以及阐明单萜类吲哚生物碱（MIA）生物合成的调控。本研究首次将ORCA 3（Octadecanoid-derivative Responsive Catharanthus AP 2-domain）基因单独或与G10 H（geraniol 10-hydroxylase）基因整合导入C.蔷薇属植物转基因长通过遗传修饰获得单独过表达ORCA 3（OR系）或共过表达G10 H和ORCA 3（GO系）的蔷薇属植物。ORCA 3过表达诱导AS、TDC、STR和D4 H转录增加，但不影响CRMYC 2和G10 H转录。在G10 H和ORCA 3共过表达下，G10 H转录物显示出显著增加。ORCA 3和G10 H过表达显著增加了异甘草酸苷、文多灵、长春质碱和阿吉马林的积累，但对脱水长春碱和长春碱水平的影响有限。基于NMR的代谢组学证实了单体吲哚生物碱在OR和GO系中的较高积累。1H NMR谱的多变量数据分析表明，与对照相比，OR和GO系中的氨基酸、有机酸、糖和苯丙素水平发生了变化。结果表明，ORCA 3和G10 H的过表达促进了MIA的合成，可能影响了植物代谢中的其他代谢途径。罗斯
In order to improve the production of the anticancer dimeric indole alkaloids in Catharanthuse roseus, much research has been dedicated to culturing cell lines, hairy roots, and efforts to elucidate the regulation of the monoterpenoid indole alkaloid (MIA) biosynthesis. In this study, the ORCA3 (Octadecanoid-derivative Responsive Catharanthus AP2-domain) gene alone or integrated with the G10H (geraniol 10-hydroxylase) gene were first introduced into C. roseus plants. Transgenic C. roseus plants overexpressing ORCA3 alone (OR lines), or co-overexpressing G10H and ORCA3 (GO lines) were obtained by genetic modification. ORCA3 overexpression induced an increase of AS, TDC, STR and D4H transcripts but did not affect CRMYC2 and G10H transcription. G10H transcripts showed a significant increase under G10H and ORCA3 co-overexpression. ORCA3 and G10H overexpression significantly increased the accumulation of strictosidine, vindoline, catharanthine and ajmalicine but had limited effects on anhydrovinblastine and vinblastine levels. NMR-based metabolomics confirmed the higher accumulation of monomeric indole alkaloids in OR and GO lines. Multivariate data analysis of 1H NMR spectra showed change of amino acid, organic acid, sugar and phenylpropanoid levels in both OR and GO lines compared to the controls. The result indicated that enhancement of MIA biosynthesis by ORCA3 and G10H overexpression might affect other metabolic pathways in the plant metabolism of C. roseus.
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影响因子：
3.8
作者：
Magnotta, Mary;Murata, Jun;De Luca, Vincenzo
通讯作者：
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影响因子：
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影响因子：
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影响因子：
7.7
作者：
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