Roles of a Cysteine Desulfhydrase LCD1 in Regulating Leaf Senescence in Tomato.
Roles of a Cysteine Desulfhydrase LCD1 in Regulating Leaf Senescence in Tomato.
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DOI：
10.3390/ijms222313078
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发表时间：
2021-12-03
影响因子：
5.6
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Hu K;Peng X;Yao G;Zhou Z;Yang F;Li W;Zhao Y;Li Y;Han Z;Chen X;Zhang H
硫化氢（H2S）是一种新的气体传递物质，在植物的生长发育和逆境响应中起着重要作用。叶片衰老是叶片发育的最后阶段。H2S产生酶L-半胱氨酸脱氢酶在番茄叶片衰老调控中的作用尚不清楚。本研究通过生理分析探讨了L-半胱氨酸脱氢酶LCD 1对番茄叶片衰老的影响。在10周番茄幼苗中，与野生型相比，LCD 1突变导致叶片衰老提前，而LCD 1过表达显著延迟叶片衰老。此外，发现LCD 1过表达延迟离体番茄叶片中暗诱导的衰老，并且lcd 1突变体表现出加速衰老。在黑暗中储存期间，观察到叶片中H2S产生的增加趋势，而LCD 1缺失减少H2S产生，与野生型对照相比，LCD 1过表达产生更多的H2S。进一步的研究表明，LCD 1过表达延迟了离体番茄叶片中暗触发的叶绿素降解和活性氧（ROS）积累，并且LCD 1过表达减弱了衰老过程中叶绿素降解基因NYC 1，PAO，PPH，SGR 1和衰老相关基因（SAGs）表达的增加，而lcd 1突变体显示出增强的衰老相关参数。相关性分析表明，叶绿素含量与H2 O2和丙二醛（MDA）含量呈负相关，与叶绿素降解相关基因和SAGs的表达也呈负相关。因此，这些发现增加了我们对H2S产生酶LCD 1在调节番茄叶片衰老中的生理功能的理解。
Hydrogen sulfide (H2S), a novel gasotransmitter in both mammals and plants, plays important roles in plant development and stress responses. Leaf senescence represents the final stage of leaf development. The role of H2S-producing enzyme L-cysteine desulfhydrase in regulating tomato leaf senescence is still unknown. In the present study, the effect of an L-cysteine desulfhydrase LCD1 on leaf senescence in tomato was explored by physiological analysis. LCD1 mutation caused earlier leaf senescence, whereas LCD1 overexpression significantly delayed leaf senescence compared with the wild type in 10-week tomato seedlings. Moreover, LCD1 overexpression was found to delay dark-induced senescence in detached tomato leaves, and the lcd1 mutant showed accelerated senescence. An increasing trend of H2S production was observed in leaves during storage in darkness, while LCD1 deletion reduced H2S production and LCD1 overexpression produced more H2S compared with the wild-type control. Further investigations showed that LCD1 overexpression delayed dark-triggered chlorophyll degradation and reactive oxygen species (ROS) accumulation in detached tomato leaves, and the increase in the expression of chlorophyll degradation genes NYC1, PAO, PPH, SGR1, and senescence-associated genes (SAGs) during senescence was attenuated by LCD1 overexpression, whereas lcd1 mutants showed enhanced senescence-related parameters. Moreover, a correlation analysis indicated that chlorophyll content was negatively correlated with H2O2 and malondialdehyde (MDA) content, and also negatively correlated with the expression of chlorophyll degradation-related genes and SAGs. Therefore, these findings increase our understanding of the physiological functions of the H2S-generating enzyme LCD1 in regulating leaf senescence in tomato.
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