Novel Salt-Tolerant Xylanase from a Mangrove-Isolated Fungus Phoma sp. MF13 and Its Application in Chinese Steamed Bread.
Novel Salt-Tolerant Xylanase from a Mangrove-Isolated Fungus Phoma sp. MF13 and Its Application in Chinese Steamed Bread.
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DOI：
10.1021/acsomega.8b00345
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发表时间：
2018-04-30
期刊：
影响因子：
4.1
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Wu J;Qiu C;Ren Y;Yan R;Ye X;Wang G
从从红树林沉积物中分离的产木聚糖酶真菌Phoma sp. MF13中克隆到了一个新的糖基水解酶家族11木聚糖酶基因xynMF13A。xynMF13A在毕赤酵母中异种表达，重组蛋白（rXynMF13A）通过ni亲和层析纯化。纯化后的rXynMF13A最适温度为45℃，最适pH为5.0。rXynMF13A表现出较高的耐盐性，在0.5 M NaCl下酶活性最高，在4 M NaCl下酶活性最高达53%。玉米芯木聚糖水解rxyynmf13a的主要产物为木糖二糖、木糖三糖、木糖四糖和木糖戊糖，未发现木糖。这些水解产物表明XynMF13A在生产低聚木糖（XOs）方面具有重要的潜力。此外，rXynMF13A还能提高馒头的比容和弹性，降低其硬度和嚼劲，有利于馒头的生产。这些结果表明，XynMF13A是一种新型木聚糖酶，在烘焙、xo生产和海鲜加工方面具有潜在的重要应用。
A novel glycosyl hydrolase family 11 xylanase gene, xynMF13A, was cloned from Phoma sp. MF13, a xylanase-producing fungus isolated from mangrove sediment. xynMF13A was heterologously expressed in Pichia pastoris, and the recombinant XynMF13A (rXynMF13A) was purified by Ni-affinity chromatography. The temperature and pH optima of purified rXynMF13A were 45 °C and pH 5.0, respectively. rXynMF13A showed a high level of salt tolerance, with maximal enzyme activity being seen at 0.5 M NaCl and as much as 53% of maximal activity at 4 M NaCl. The major rXynMF13A hydrolysis products from corncob xylan were xylobiose, xylotriose, xylotetraose, and xylopentaose, but no xylose was found. These hydrolysis products suggest an important potential for XynMF13A in the production of xylooligosaccharides (XOs). Furthermore, rXynMF13A had beneficial effects on Chinese steamed bread production, by increasing specific volume and elasticity while decreasing hardness and chewiness. These results demonstrate XynMF13A to be a novel xylanase with potentially significant applications in baking, XOs production, and seafood processing.
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