Requirements for the Packaging of Geminivirus Circular Single-Stranded DNA: Effect of DNA Length and Coat Protein Sequence.
Requirements for the Packaging of Geminivirus Circular Single-Stranded DNA: Effect of DNA Length and Coat Protein Sequence.
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DOI：
10.3390/v12111235
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发表时间：
2020-10-30
期刊：
影响因子：
--
通讯作者：
Lomonossoff GP
中科院分区：
文献类型：
--
作者：
Saunders K;Richardson J;Lawson DM;Lomonossoff GP
双生病毒颗粒由一对孪生等轴结构组成，具有病毒学世界中最独特的衣壳之一。直到最近，关于这些结构是如何产生的信息还很少。为了解决这个问题，我们开发了一个系统，以产生衣壳结构后，双生病毒外壳蛋白和复制环状单链DNA（cssDNA）的基因构建体渗透到植物叶片的交付。不同双生病毒属的外壳蛋白对双生病毒属的cssDNA的转座作用显示发生在全长分子而不是半长分子。在该表达系统中还产生了与双生衣壳结构不同的双衣壳结构。通过增加衣壳化的cssDNA的长度，产生由直的、弯曲的和浓缩的形式组成的三重双生衣壳结构。产生的直双生三重结构在形态上与来自秘鲁的马铃薯感染病毒记录的那些相似。这些发现表明，双核苷酸化DNA的长度控制双生病毒颗粒的大小和稳定性。
Geminivirus particles, consisting of a pair of twinned isometric structures, have one of the most distinctive capsids in the virological world. Until recently, there was little information as to how these structures are generated. To address this, we developed a system to produce capsid structures following the delivery of geminivirus coat protein and replicating circular single-stranded DNA (cssDNA) by the infiltration of gene constructs into plant leaves. The transencapsidation of cssDNA of the Begomovirus genus by coat protein of different geminivirus genera was shown to occur with full-length but not half-length molecules. Double capsid structures, distinct from geminate capsid structures, were also generated in this expression system. By increasing the length of the encapsidated cssDNA, triple geminate capsid structures, consisting of straight, bent and condensed forms were generated. The straight geminate triple structures generated were similar in morphology to those recorded for a potato-infecting virus from Peru. These finding demonstrate that the length of encapsidated DNA controls both the size and stability of geminivirus particles.
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影响因子：
16.6
作者：
Hesketh EL;Saunders K;Fisher C;Potze J;Stanley J;Lomonossoff GP;Ranson NA
通讯作者：
Ranson NA
影响因子：
3.7
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Bennett, Antonette;Rodriguez, David;Agbandje-McKenna, Mavis
通讯作者：
Agbandje-McKenna, Mavis
影响因子：
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通讯作者：
Lapidot, Moshe
影响因子：
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Ito, Takuya;Kimbara, Junji;Ikegami, Masato
通讯作者：
Ikegami, Masato
影响因子：
3.7
作者：
BRIDDON, RW;WATTS, J;STANLEY, J
通讯作者：
STANLEY, J