Resistance to Botrytis cinerea in Solanum lycopersicoides involves widespread transcriptional reprogramming.
Resistance to Botrytis cinerea in Solanum lycopersicoides involves widespread transcriptional reprogramming.
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DOI：
10.1186/1471-2164-15-334
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发表时间：
2014-05-03
期刊：
影响因子：
4.4
通讯作者：
Bluhm BH
中科院分区：
文献类型：
--
作者：
Smith JE;Mengesha B;Tang H;Mengiste T;Bluhm BH
番茄（Solanum lycopersicum）是世界上最重要的蔬菜作物之一，对灰葡萄孢（Botrytis cinerea）和茄链格孢（Alternaria solani）等坏死型病原真菌高度敏感。通过常规育种提高抗性受到抗性种质资源短缺和难以将抗性渗入无连锁阻力的优良种质的阻碍。本研究的目的是探索野生茄属植物之间的自然变异，以确定新的抗坏死营养型真菌的来源，并剖析抗B的机制。灰叶在8个野生种中评价了对B的抗性。cinerea和A. solani，S.类番茄对这两种病原体的抗性水平最高。抗B。灰霉病表现为抑制病原菌生长。通过下一代RNA测序和S.在病原体感染期间，分析番茄类番茄的转录组、基因表达的变化。对B的回应。在灰霉病中，差异表达的转录物分为四类：表达迅速增加然后迅速减少的基因，表达迅速增加并稳定的基因，表达持续增加的基因，以及表达减少的基因。基于同源性的检索也鉴定了有限数量的高表达B。cinerea基因几乎是在被B感染后立即。cinerea、灰毛藓S.番茄类抑制光合作用和参与生长、能量产生和对刺激的响应的代谢过程，并同时诱导各种防御相关基因，包括病程相关蛋白1（PR 1）、β-1，3-葡聚糖酶（葡聚糖酶）和枯草杆菌蛋白酶样蛋白酶，表明优先向防御转移。此外，聚类分析揭示了新的，未表征的基因，可能在防御坏死营养型真菌病原体在S。类番茄。对S.番茄感染B.灰霉病揭示了诱导的起始和强度的差异，从而阐明了解释易感性增加的潜在机制。此外，代谢途径分析确定了推定的防御相关类别的次级代谢产物。综上所述，本研究为茄科植物对坏死型病原真菌的抗性研究提供了新的思路，同时也为茄科植物对坏死型病原真菌的抗性研究提供了新的序列资源。类番茄。本文的在线版本（doi：10.1186/1471-2164-15-334）包含补充材料，可供授权用户使用。
Tomato (Solanum lycopersicum), one of the world’s most important vegetable crops, is highly susceptible to necrotrophic fungal pathogens such as Botrytis cinerea and Alternaria solani. Improving resistance through conventional breeding has been hampered by a shortage of resistant germplasm and difficulties in introgressing resistance into elite germplasm without linkage drag. The goal of this study was to explore natural variation among wild Solanum species to identify new sources of resistance to necrotrophic fungi and dissect mechanisms underlying resistance against B. cinerea. Among eight wild species evaluated for resistance against B. cinerea and A. solani, S. lycopersicoides expressed the highest levels of resistance against both pathogens. Resistance against B. cinerea manifested as containment of pathogen growth. Through next-generation RNA sequencing and de novo assembly of the S. lycopersicoides transcriptome, changes in gene expression were analyzed during pathogen infection. In response to B. cinerea, differentially expressed transcripts grouped into four categories: genes whose expression rapidly increased then rapidly decreased, genes whose expression rapidly increased and plateaued, genes whose expression continually increased, and genes with decreased expression. Homology-based searches also identified a limited number of highly expressed B. cinerea genes. Almost immediately after infection by B. cinerea, S. lycopersicoides suppressed photosynthesis and metabolic processes involved in growth, energy generation, and response to stimuli, and simultaneously induced various defense-related genes, including pathogenesis-related protein 1 (PR1), a beta-1,3-glucanase (glucanase), and a subtilisin-like protease, indicating a shift in priority towards defense. Moreover, cluster analysis revealed novel, uncharacterized genes that may play roles in defense against necrotrophic fungal pathogens in S. lycopersicoides. The expression of orthologous defense-related genes in S. lycopersicum after infection with B. cinerea revealed differences in the onset and intensity of induction, thus illuminating a potential mechanism explaining the increased susceptibility. Additionally, metabolic pathway analyses identified putative defense-related categories of secondary metabolites. In sum, this study provided insight into resistance against necrotrophic fungal pathogens in the Solanaceae, as well as novel sequence resources for S. lycopersicoides. The online version of this article (doi: 10.1186/1471-2164-15-334) contains supplementary material, which is available to authorized users.
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