Isolation of microplastics in biota-rich seawater samples and marine organisms.

Isolation of microplastics in biota-rich seawater samples and marine organisms.
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DOI:
10.1038/srep04528
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发表时间:
2014-03-31
期刊:
影响因子:
4.6
通讯作者:
Galloway TS
Galloway TS
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Cole M;Webb H;Lindeque PK;Fileman ES;Halsband C;Galloway TS

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微塑料垃圾是地球仪水生系统中普遍存在的污染物。一系列海洋生物有能力摄入微塑料,对健康产生不利影响。开发方法来准确量化生产性海洋沃茨中的微塑料,以及那些被海洋生物内化的微塑料,越来越重要。在这里,我们研究了使用酸,碱和酶消化技术在海洋表面拖网生物材料矿化中的功效,以揭示任何存在的微塑料。我们优化的酶促方案可以消化富含维生素B1的海水样品中存在的>97%(按重量计)的材料,而不会破坏任何存在的微塑料碎片。在应用该方法复制英吉利海峡西部的海洋样品时,我们确定了0.27 m−3的微塑料。该方案进一步用于在实验室条件下提取海洋浮游动物摄入的微塑料。我们的研究结果表明,酶消化可以帮助检测海水样品和海洋生物群中的微塑料碎片。
Microplastic litter is a pervasive pollutant present in aquatic systems across the globe. A range of marine organisms have the capacity to ingest microplastics, resulting in adverse health effects. Developing methods to accurately quantify microplastics in productive marine waters, and those internalized by marine organisms, is of growing importance. Here we investigate the efficacy of using acid, alkaline and enzymatic digestion techniques in mineralizing biological material from marine surface trawls to reveal any microplastics present. Our optimized enzymatic protocol can digest >97% (by weight) of the material present in plankton-rich seawater samples without destroying any microplastic debris present. In applying the method to replicate marine samples from the western English Channel, we identified 0.27 microplastics m−3. The protocol was further used to extract microplastics ingested by marine zooplankton under laboratory conditions. Our findings illustrate that enzymatic digestion can aid the detection of microplastic debris within seawater samples and marine biota.
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