An updated view on horseradish peroxidases: recombinant production and biotechnological applications.
An updated view on horseradish peroxidases: recombinant production and biotechnological applications.
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DOI：
10.1007/s00253-014-6346-7
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发表时间：
2015-02
影响因子：
5
通讯作者：
Glieder, Anton
中科院分区：
文献类型：
--
作者：
Krainer, Florian W.;Glieder, Anton
关键词：
辣根过氧化物酶几个世纪以来一直是科学研究的主题。它已被彻底用作报告酶在诊断和组织化学，并仍然在这些应用中发挥着重要作用。辣根过氧化物酶在植物中的作用及其催化机制已被广泛研究。然而，在其重组生产方面进展甚微。直到现在，辣根过氧化物酶的商业制剂仍然是从植物根部分离的。这些制剂通常是各种同工酶的混合物，迄今为止仅描述了其中的一小部分。这些混合分离物中的同工酶的组成受到无法控制的环境条件的影响。如今，辣根过氧化物酶由于其在医学、生命科学和癌症治疗、生物传感器系统、生物修复和生物催化中的生物技术领域的广泛应用而重新引起人们的兴趣。这些医学和商业相关的应用，最近发现的新的天然同工酶具有不同的生化特性，以及重组生产的挑战，使这种酶特别感兴趣的未来生物技术的解决方案。因此，我们回顾了以前的研究以及目前的发展与生物技术的重点新的应用和主要剩余的生物技术的挑战，有效的重组生产辣根过氧化物酶。
Horseradish peroxidase has been the subject of scientific research for centuries. It has been used exhaustively as reporter enzyme in diagnostics and histochemistry and still plays a major role in these applications. Numerous studies have been conducted on the role of horseradish peroxidase in the plant and its catalytic mechanism. However, little progress has been made in its recombinant production. Until now, commercial preparations of horseradish peroxidase are still isolated from plant roots. These preparations are commonly mixtures of various isoenzymes of which only a small fraction has been described so far. The composition of isoenzymes in these mixed isolates is subjected to uncontrollable environmental conditions. Nowadays, horseradish peroxidase regains interest due to its broad applicability in the fields of medicine, life sciences, and biotechnology in cancer therapy, biosensor systems, bioremediation, and biocatalysis. These medically and commercially relevant applications, the recent discovery of new natural isoenzymes with different biochemical properties, as well as the challenges in recombinant production render this enzyme particularly interesting for future biotechnological solutions. Therefore, we reviewed previous studies as well as current developments with biotechnological emphasis on new applications and the major remaining biotechnological challenge—the efficient recombinant production of horseradish peroxidase enzymes.
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影响因子：
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作者：
da Silva MR;de Sá LR;Russo C;Scio E;Ferreira-Leitão VS
通讯作者：
Ferreira-Leitão VS
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影响因子：
2.7
作者：
Cao, Xiaodan;Chen, Chao;Wang, Ping
通讯作者：
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影响因子：
2.9
作者：
BJELLQVIST, B;BASSE, B;CELIS, JE
通讯作者：
CELIS, JE
影响因子：
2.9
作者：
BJELLQVIST, B;HUGHES, GJ;HOCHSTRASSER, D
通讯作者：
HOCHSTRASSER, D
影响因子：
2.9
作者：
Barber, Janet E. B.;Damry, Adam M.;Chica, Roberto A.
通讯作者：
Chica, Roberto A.