Fungal mycelia and bacterial thiamine establish a mutualistic growth mechanism.
Fungal mycelia and bacterial thiamine establish a mutualistic growth mechanism.
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DOI：
10.26508/lsa.202000878
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发表时间：
2020-12
影响因子：
4.4
通讯作者：
Takeshita N
中科院分区：
文献类型：
--
作者：
Abeysinghe G;Kuchira M;Kudo G;Masuo S;Ninomiya A;Takahashi K;Utada AS;Hagiwara D;Nomura N;Takaya N;Obana N;Takeshita N
细菌细胞沿着真菌高速公路行进，并向真菌支付硫胺素作为通行费。同时空间和代谢相互作用，在通信细菌和真菌物种建立一个互惠共生，有利于他们获得一个环境生态位和营养。物理空间和营养的排他性是生物生存的先决条件，但少数物种已经能够发展互利的策略，使它们能够共同生活。在这里，我们发现了丝状真菌构巢曲霉和细菌枯草芽孢杆菌之间的互惠机制。与真菌共培养的细菌细胞利用鞭毛沿着菌丝运动，并随着真菌菌落的扩大而进一步扩散，表明真菌菌丝为细菌的迁移、扩散和增殖提供了空间。转录组学、遗传学、分子质量和成像分析表明，细菌到达菌丝体边缘并向生长的菌丝提供硫胺素，从而促进菌丝生长。稳定同位素标记直接证明了硫胺素从细菌转移到硫胺素非营养缺陷型真菌。同时空间和代谢的相互作用表明，在这项研究中揭示了一种互利共生，有利于通信真菌和细菌物种，以获得一个环境生态位和营养，分别。
Bacterial cells travel along fungal highway and pay thiamine as a toll to the fungus. Simultaneous spatial and metabolic interactions in communicating bacterial and fungal species establish a mutualism that facilitates them to obtain an environmental niche and nutrient. Exclusivity in physical spaces and nutrients is a prerequisite for survival of organisms, but a few species have been able to develop mutually beneficial strategies that allow them to co-habit. Here, we discovered a mutualistic mechanism between filamentous fungus, Aspergillus nidulans, and bacterium, Bacillus subtilis. The bacterial cells co-cultured with the fungus traveled along mycelia using their flagella and dispersed farther with the expansion of fungal colony, indicating that the fungal mycelia supply space for bacteria to migrate, disperse, and proliferate. Transcriptomic, genetic, molecular mass, and imaging analyses demonstrated that the bacteria reached the mycelial edge and supplied thiamine to the growing hyphae, which led to a promotion of hyphal growth. The thiamine transfer from bacteria to the thiamine non-auxotrophic fungus was directly demonstrated by stable isotope labeling. The simultaneous spatial and metabolic interactions demonstrated in this study reveal a mutualism that facilitates the communicating fungal and bacterial species to obtain an environmental niche and nutrient, respectively.
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影响因子：
16.6
作者：
Jurgenson CT;Begley TP;Ealick SE
通讯作者：
Ealick SE
DOI：
10.1038/s41396-018-0095-z
发表时间：
2018-06
期刊：
The ISME journal
影响因子：
--
作者：
Jiang X;Zerfaß C;Feng S;Eichmann R;Asally M;Schäfer P;Soyer OS
通讯作者：
Soyer OS
影响因子：
5.1
作者：
Benoit, Isabelle;van den Esker, Marielle H.;Kovacs, Akos T.
通讯作者：
Kovacs, Akos T.
影响因子：
9.7
作者：
Osono, T;Ono, Y;Takeda, H
通讯作者：
Takeda, H
影响因子：
1.6
作者：
Shimizu, Motoyuki;Masuo, Shunsuke;Takaya, Naoki
通讯作者：
Takaya, Naoki