Neuropeptide substance P improves osteoblastic and angiogenic differentiation capacity of bone marrow stem cells in vitro.
Neuropeptide substance P improves osteoblastic and angiogenic differentiation capacity of bone marrow stem cells in vitro.
复制标题
神经肽 P 物质在体外提高骨髓干细胞的成骨细胞和血管生成分化能力
DOI：
10.1155/2014/596023
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发表时间：
2014
影响因子：
--
通讯作者：
Jin D
中科院分区：
文献类型：
--
作者：
Fu S;Mei G;Wang Z;Zou ZL;Liu S;Pei GX;Bi L;Jin D
我们的前期工作表明，在构建血管神经化骨时，植入感觉神经或血管束可以促进组织工程骨的成骨。这种现象可以用神经肽的调节功能来解释。神经肽P物质（SP）通过刺激骨髓干细胞（BMSCs）的增殖和分化促进骨生长。然而，在BMSC分化的背景下，Wnt信号传导和SP机制之间的关联尚未有先前的研究。我们的研究结果表明，SP可以通过激活Wnt信号通路的基因和蛋白表达以及通过转位β-catenin来促进BMSCs的分化，这可以被Wnt信号通路阻断剂或NK-1拮抗剂抑制。SP还可增加骨形态发生蛋白-2（BMP-2）的生长因子水平。此外，SP可增强BMSCs的迁移能力，并研究了SP对血管内皮生长因子（VEGF）表达的促进作用。结论：SP可通过Wnt途径诱导BMSC向成骨细胞分化，促进BMSC的血管生成能力。这些结果表明，血管化和神经化的组织工程构建体可能是可行的，用于骨组织工程策略。
Our previous work showed that implanting a sensory nerve or vascular bundle when constructing vascularized and neurotized bone could promote bone osteogenesis in tissue engineering. This phenomenon could be explained by the regulatory function of neuropeptides. Neuropeptide substance P (SP) has been demonstrated to contribute to bone growth by stimulating the proliferation and differentiation of bone marrow stem cells (BMSCs). However, there have been no prior studies on the association between Wnt signaling and the mechanism of SP in the context of BMSC differentiation. Our results have shown that SP could enhance the differentiation of BMSCs by activating gene and protein expression via the Wnt pathway and by translocating β-catenin, which can be inhibited by Wnt signaling blocker treatment or by the NK-1 antagonist. SP could also increase the growth factor level of bone morphogenetic protein-2 (BMP-2). Additionally, SP could enhance the migration ability of BMSCs, and the promotion of vascular endothelial growth factor (VEGF) expression by SP has been studied. In conclusion, SP could induce osteoblastic differentiation via the Wnt pathway and promote the angiogenic ability of BMSCs. These results indicate that a vascularized and neurotized tissue-engineered construct could be feasible for use in bone tissue engineering strategies.
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影响因子：
4
作者：
Hoppler, Stefan;Kavanagh, Claire Louise
通讯作者：
Kavanagh, Claire Louise
DOI：
10.1097/01.moo.0000132242.81060.4a
发表时间：
2004-08-01
影响因子：
1.6
作者：
Nussenbaum, Brian;Teknos, Theodoros N;Chepeha, Douglas B
通讯作者：
Chepeha, Douglas B
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3.6
作者：
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通讯作者：
Vilamitjana-Amedee, J
影响因子：
4.1
作者：
Georgiou, Kristen R.;King, Tristan J.;Xian, Cory J.
通讯作者：
Xian, Cory J.
影响因子：
11.2
作者：
Kurayoshi, Manabu;Oue, Naohide;Kikuchi, Akira
通讯作者：
Kikuchi, Akira