A communal bacterial adhesin anchors biofilm and bystander cells to surfaces.
A communal bacterial adhesin anchors biofilm and bystander cells to surfaces.
复制标题
DOI：
10.1371/journal.ppat.1002210
复制
发表时间：
2011-08
期刊：
影响因子：
6.7
通讯作者：
Watnick PI
中科院分区：
文献类型：
--
作者：
Absalon C;Van Dellen K;Watnick PI
虽然生物膜基质的胞外多糖成分已被深入研究，但对基质相关蛋白的了解却少之又少。为了更好地了解这些蛋白质的作用，我们对霍乱弧菌生物膜基质进行了蛋白质组分析。在这里，我们展示了两种基质相关蛋白 Bap1 和 RbmA 在生物膜基质中发挥不同的作用。 RbmA 增强细胞间附着。相比之下，Bap1 集中在表面，用于锚定生物膜并招募尚未采取固着生活方式的细胞。这是生物膜衍生的、共同合成的调理膜的第一个例子，它可以稳定多层生物膜与表面的结合，并促进浮游旁观者招募到底层。这些研究定义了生物膜基质中蛋白质的空间和功能分化的新范例，并为细菌在多层生物膜的维持和扩张中的合作提供了证据。细菌多层生物膜由基质封闭的细胞组成，细胞彼此附着形成大的聚集体。这些骨料的底部可以附着在有生命或无生命的表面上。生物膜基质通常含有至少一种胞外多糖成分，并且还可以含有蛋白质和DNA。虽然人们对革兰氏阴性生物膜基质的胞外多糖成分了解很多，但对生物膜基质蛋白的功能知之甚少。我们假设生物膜基质可能含有具有多种功能的蛋白质。因此，我们对革兰氏阴性菌霍乱弧菌的生物膜基质进行了首次蛋白质组分析。随后我们重点关注 Bap1 和 RbmA，这两种生物膜基质中丰富的蛋白质。 RbmA 可增强细胞间相互作用，被发现均匀分布在生物膜中。相比之下，共同合成的 Bap1 集中在生物膜-表面界面，并稳定了多层生物膜与表面的结合。此外，纯化的 Bap1 的添加增加了自由游动细胞对表面的附着。这些研究为生物膜基质中蛋白质的空间和功能分化提供了证据，并表明细菌在稳定多层生物膜表面缔合和招募新成员方面的合作。
While the exopolysaccharide component of the biofilm matrix has been intensively studied, much less is known about matrix-associated proteins. To better understand the role of these proteins, we undertook a proteomic analysis of the V. cholerae biofilm matrix. Here we show that the two matrix-associated proteins, Bap1 and RbmA, perform distinct roles in the biofilm matrix. RbmA strengthens intercellular attachments. In contrast, Bap1 is concentrated on surfaces where it serves to anchor the biofilm and recruit cells not yet committed to the sessile lifestyle. This is the first example of a biofilm-derived, communally synthesized conditioning film that stabilizes the association of multilayer biofilms with a surface and facilitates recruitment of planktonic bystanders to the substratum. These studies define a novel paradigm for spatial and functional differentiation of proteins in the biofilm matrix and provide evidence for bacterial cooperation in maintenance and expansion of the multilayer biofilm. The bacterial multilayer biofilm consists of matrix-enclosed cells attached to each other to form large aggregates. The base of these aggregates may be attached to a living or non-living surface. The biofilm matrix most often contains at least one exopolysaccharide component and may also contain protein and DNA. While much is known about the exopolysaccharide component of the Gram-negative biofilm matrix, little is known about the function of biofilm matrix proteins. We hypothesized that the biofilm matrix might harbor proteins with diverse functions. Therefore, we undertook the first proteomic analysis of the biofilm matrix of a Gram-negative bacterium, V. cholerae. We subsequently focused on Bap1 and RbmA, two proteins that are abundant in the biofilm matrix. RbmA, which strengthens intercellular interactions, was found to be evenly distributed in the biofilm. In contrast, communally synthesized Bap1 was concentrated at the biofilm-surface interface and stabilized the association of the multilayer biofilm with the surface. Furthermore, the addition of purified Bap1 increased attachment of free-swimming cells to a surface. These studies provide evidence for spatial and functional differentiation of proteins in the biofilm matrix and suggest bacterial cooperation in stabilization of multilayer biofilm surface association and recruitment of new members.
登录
查看更多内容
影响因子：
3.6
作者：
Hammer, BK;Bassler, BL
通讯作者：
Bassler, BL
影响因子：
3.6
作者：
Lathem, WW;Grys, TE;Welch, RA
通讯作者：
Welch, RA
DOI：
10.1073/pnas.2334614100
发表时间：
2003-11-25
影响因子：
11.1
作者：
Kierek, K;Watnick, PI
通讯作者：
Watnick, PI
影响因子：
2.8
作者：
Berg, Thorsten;Schild, Stefan;Reidl, Joachim
通讯作者：
Reidl, Joachim
影响因子：
3.6
作者：
Borlee BR;Goldman AD;Murakami K;Samudrala R;Wozniak DJ;Parsek MR
通讯作者：
Parsek MR