Noninvasive optical imaging of nanomedicine biodistribution.
Noninvasive optical imaging of nanomedicine biodistribution.
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DOI：
10.1021/nn303955n
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发表时间：
2013-01-22
期刊：
影响因子：
17.1
通讯作者：
Lammers T
中科院分区：
文献类型：
--
作者：
Kunjachan S;Gremse F;Theek B;Koczera P;Pola R;Pechar M;Etrych T;Ulbrich K;Storm G;Kiessling F;Lammers T
纳米药物是亚微米大小的载体材料，旨在改善静脉注射（化学）治疗剂的生物分布。近年来，纳米医学领域越来越多地利用光学成像（OI）技术来监测生物分布和靶点积累。然而，到目前为止，由于光线穿透有限（2D荧光反射成像；FRI），以及无法准确地将荧光信号分配到非浅表器官（3D荧光分子断层扫描；FMT），利用OI对纳米药物生物分布进行纵向评估是不可能的。利用高分辨率微计算机断层扫描（μCT）和FMT的结合，我们在这里着手建立一种混合成像方案，用于非侵入性可视化和量化近红外荧光团标记的纳米药物在浅表肿瘤以外组织中的积累。为此，用Dy750标记基于hpma的高分子药物载体，利用FMT分析其生物分布和肿瘤蓄积，并将所得数据集与解剖μCT数据集融合，在解剖μCT数据集中对多个不同的生理相关器官进行预分割。验证了三维器官分割的鲁棒性，并将三维CT-FMT结果与标准三维FMT和二维FRI结果进行了比较，结果令人信服地表明，解剖μCT与分子FMT相结合可以促进纳米药物生物分布的非侵入性评估。
Nanomedicines are submicrometer-sized carrier materials designed to improve the biodistribution of i.v. administered (chemo-) therapeutic agents. In recent years, ever more efforts in the nanomedicine field have employed optical imaging (OI) techniques to monitor biodistribution and target site accumulation. Thus far, however, the longitudinal assessment of nanomedicine biodistribution using OI has been impossible, due to limited light penetration (in case of 2D fluorescence reflectance imaging; FRI), and to the inability to accurately allocate fluorescent signals to non-superficial organs (in case of 3D fluorescence molecular tomography; FMT). Using a combination of high-resolution micro-computed tomography (μCT) and FMT, we have here set out to establish a hybrid imaging protocol for non-invasively visualizing and quantifying the accumulation of near-infrared fluorophore-labeled nanomedicines in tissues other than superficial tumors. To this end, HPMA-based polymeric drug carriers were labeled with Dy750, their biodistribution and tumor accumulation were analyzed using FMT, and the resulting data sets were fused with anatomical μCT data sets in which several different physiologically relevant organs were pre-segmented. The robustness of 3D organ segmentation was validated, and the results obtained using 3D CT-FMT were compared to those obtained upon standard 3D FMT and 2D FRI. Our findings convincingly demonstrate that combining anatomical μCT with molecular FMT facilitates the non-invasive assessment of nanomedicine biodistribution.
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影响因子：
18.3
作者：
Mulder, Willem J. M.;Strijkers, Gustav J.;Van Tilborg, Geralda A. F.;Cormode, David P.;Fayad, Zahi A.;Nicolay, Klaas
通讯作者：
Nicolay, Klaas
影响因子：
46.9
作者：
Ntziachristos, V;Ripoll, J;Weissleder, R
通讯作者：
Weissleder, R
影响因子：
10.8
作者：
Lee S;Ryu JH;Park K;Lee A;Lee SY;Youn IC;Ahn CH;Yoon SM;Myung SJ;Moon DH;Chen X;Choi K;Kwon IC;Kim K
通讯作者：
Kim K
影响因子：
16.1
作者：
Janib, Siti M.;Moses, Ara S.;MacKay, J. Andrew
通讯作者：
MacKay, J. Andrew
影响因子：
78.8
作者：
Jain, Rakesh K.;Stylianopoulos, Triantafyllos
通讯作者：
Stylianopoulos, Triantafyllos