Nanoparticulate assemblies of amphiphiles and diagnostically active materials for multimodality imaging.
Nanoparticulate assemblies of amphiphiles and diagnostically active materials for multimodality imaging.
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DOI：
10.1021/ar800223c
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发表时间：
2009-07-21
影响因子：
18.3
通讯作者：
Nicolay, Klaas
中科院分区：
文献类型：
--
作者：
Mulder, Willem J. M.;Strijkers, Gustav J.;Van Tilborg, Geralda A. F.;Cormode, David P.;Fayad, Zahi A.;Nicolay, Klaas
现代医学极大地受益于最近成像技术的巨大进步。通过在分子水平上操纵的相互作用来观察生理事件，可以提供对生物体功能（和功能障碍）的独特见解。过去十年中纳米颗粒分子成像剂的发展取得了巨大进步，使得对许多新型化合物的特异性、药代动力学特征、生物分布和治疗功效进行非侵入性成像成为可能。几种类型的纳米颗粒已被证明可用于生物医学目的，包括无机纳米晶体，如氧化铁、金和量子纳米颗粒。 点。此外，天然纳米颗粒，如病毒、脂蛋白或脱铁铁蛋白，以及由无机和天然纳米颗粒组成的混合纳米结构，已得到广泛应用。然而，用于生物医学目的研究最多的纳米颗粒平台是脂质聚集体，例如脂质体纳米颗粒、胶束和微乳液。它们相对容易的制备和功能化，以及组合多个两亲部分的现成合成能力，是它们受欢迎的最重要原因。基于脂质的纳米颗粒平台允许包含多种成像剂，从荧光分子到螯合金属和纳米晶体。近年来，我们创建了多种用于分子成像的多功能基于脂质的纳米颗粒；许多能够与多种成像技术一起使用（即具有多模态成像能力）。这些纳米颗粒的大小、形态和生物标记物的特异性不同。在本报告中，我们讨论了五种不同颗粒的开发和表征：脂质体、胶束、纳米晶体胶束、脂质包被二氧化硅和纳米晶体高密度脂蛋白 (HDL)。我们还展示了它们在多模态分子成像中的应用，主要关注磁共振成像 (MRI)、光学技术和透射电子显微镜 (TEM)。讨论了纳米颗粒的功能化及其药代动力学的调节。展示了它们在癌症和心血管疾病关键过程的分子成像中的应用。最后，我们讨论了最近的进展，其中内源性纳米颗粒 HDL 被修饰以携带不同的诊断活性纳米晶体核心，从而能够在实验性动脉粥样硬化中对巨噬细胞进行多模态成像。不同造影剂平台的多模态特征已被证明对于验证目的和理解不同级别（从整个生物体到细胞器）粒子与靶标相互作用的机制非常有价值。
Modern medicine has greatly benefited from recent dramatic improvements in imaging techniques. The observation of physiological events through interactions manipulated at the molecular level offers unique insight into the function (and dysfunction) of the living organism. The tremendous advances in the development of nanoparticulate molecular imaging agents over the past decade have made it possible to noninvasively image the specificity, pharmacokinetic profiles, biodistribution, and therapeutic efficacy of many novel compounds.Several types of nanoparticles have demonstrated utility for biomedical purposes, including inorganic nanocrystals, such as iron oxide, gold, and quantum dots. Moreover, natural nanoparticles, such as viruses, lipoproteins, or apoferritin, as well as hybrid nanostructures composed of inorganic and natural nanoparticles, have been applied broadly. However, among the most investigated nanoparticle platforms for biomedical purposes are lipidic aggregates, such as liposomal nanoparticles, micelles, and microemulsions. Their relative ease of preparation and functionalization, as well as the ready synthetic ability to combine multiple amphiphilic moieties, are the most important reasons for their popularity. Lipid-based nanoparticle platforms allow the inclusion of a variety of imaging agents, ranging from fluorescent molecules to chelated metals and nanocrystals.In recent years, we have created a variety of multifunctional lipid-based nanoparticles for molecular imaging; many are capable of being used with more than one imaging technique (that is, with multimodal imaging ability). These nanoparticles differ in size, morphology, and specificity for biological markers. In this Account, we discuss the development and characterization of five different particles: liposomes, micelles, nanocrystal micelles, lipid-coated silica, and nanocrystal high-density lipoprotein (HDL). We also demonstrate their application for multimodal molecular imaging, with the main focus on magnetic resonance imaging (MRI), optical techniques, and transmission electron microscopy (TEM). The functionalization of the nanoparticles and the modulation of their pharmacokinetics are discussed. Their application for molecular imaging of key processes in cancer and cardiovascular disease are shown. Finally, we discuss a recent development in which the endogenous nanoparticle HDL was modified to carry different diagnostically active nanocrystal cores to enable multimodal imaging of macrophages in experimental atherosclerosis. The multimodal characteristics of the different contrast agent platforms have proven to be extremely valuable for validation purposes and for understanding mechanisms of particle−target interaction at different levels, ranging from the entire organism down to cellular organelles.
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影响因子：
10.8
作者：
Huang, Xinlei;Bronstein, Lyudmila M.;Dragnea, Bogdan
通讯作者：
Dragnea, Bogdan
影响因子：
3.3
作者：
Mulder, Willem J. M.;Strijkers, Gustav J.;Fayad, Zahi A.
通讯作者：
Fayad, Zahi A.
影响因子：
10.8
作者：
Cormode DP;Skajaa T;van Schooneveld MM;Koole R;Jarzyna P;Lobatto ME;Calcagno C;Barazza A;Gordon RE;Zanzonico P;Fisher EA;Fayad ZA;Mulder WJ
通讯作者：
Mulder WJ
影响因子：
56.9
作者：
Dubertret, B;Skourides, P;Libchaber, A
通讯作者：
Libchaber, A
影响因子：
15
作者：
Frias, JC;Williams, KJ;Fayad, ZA
通讯作者：
Fayad, ZA