Anti-vascular endothelial growth factor treatment decreases bladder pain in cyclophosphamide cystitis: a Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network animal model study.
Anti-vascular endothelial growth factor treatment decreases bladder pain in cyclophosphamide cystitis: a Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network animal model study.
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抗血管内皮生长因子治疗可减轻环磷酰胺膀胱炎的膀胱疼痛：慢性盆腔疼痛研究的多学科方法 (MAPP) 研究网络动物模型研究
DOI：
10.1111/bju.13924
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发表时间：
2017-10
影响因子：
4.5
通讯作者：
Gereau RW 4th
中科院分区：
文献类型：
--
作者：
Lai HH;Shen B;Vijairania P;Zhang X;Vogt SK;Gereau RW 4th
研究用抗VEGF（血管内皮生长因子）中和抗体治疗是否可以减轻环磷酰胺（CTX）膀胱炎小鼠膀胱疼痛模型中的疼痛和排尿功能障碍。成年雌性小鼠接受抗VEGF中和抗体（10 mg/kg腹膜内B20-4.1.1 VEGF mAb）或盐水（对照）预处理，然后接受顺铂（150 mg/kg腹膜内）诱导急性膀胱炎。通过将von Frey细丝应用于骨盆区域来评估骨盆伤害性反应。使用空斑试验评估自发排尿。与对照组（生理盐水）相比，全身性抗VEGF中和抗体治疗显著降低了盆腔对膀胱炎的伤害性反应。在抗VEGF预处理组中，在子宫内膜异位症后5小时，通过von Frey细丝的曲线下面积（AUC）测量的盆腔超敏反应显著增加（p=0.0035）。然而，到盆腔炎后48和96小时，与盆腔炎后5小时时间点相比，盆腔超敏反应分别降低了54%和47%，与基线相比不再有显著差异（分别为p=0.22和0.17）。两个治疗前组之间的排尿频率和平均排尿量无差异。在膀胱疼痛的膀胱炎模型中，用抗VEGF中和抗体全身阻断VEGF信号传导有效减轻骨盆/膀胱疼痛。我们的数据支持进一步研究使用抗VEGF抗体来管理膀胱疼痛或内脏疼痛。
To investigate whether treatment with anti-VEGF (vascular endothelial growth factor) neutralizing antibodies can reduce pain and voiding dysfunction in the cyclophosphamide (CYP) cystitis model of bladder pain in mice. Adult female mice received anti-VEGF neutralizing antibodies (10 mg/kg intraperitoneal B20-4.1.1 VEGF mAb) or saline (control) pre-treatment, followed by CYP (150 mg/kg intraperitoneal) to induce acute cystitis. Pelvic nociceptive responses were assessed by applying von Frey filaments to the pelvic area. Spontaneous micturition was assessed using the void spot assay. Systemic anti-VEGF neutralizing antibodies treatment significantly reduced the pelvic nociceptive response to CYP cystitis compared to control (saline). In the anti-VEGF pre-treatment group, there was a significant increase in pelvic hypersensitivity measured by the area under the curve (AUC) with von Frey filaments at 5 hours post-CYP (p=0.0035). However by 48 and 96 hours post-CYP, the pelvic hypersensitivity have reduced by 54% and 47% respectively compared to the 5 hours post-CYP time point, and were no longer significantly different from the baseline (p=0.22 and 0.17 respectively). There was no difference in urinary frequency and mean voided volume between the two pre-treatment groups. Systemic blockade of VEGF signaling with anti-VEGF neutralizing antibodies was effective in reducing pelvic/bladder pain in the CYP cystitis model of bladder pain. Our data support the further investigation of the use of anti-VEGF antibodies to manage bladder pain or visceral pain.
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影响因子：
4.5
作者：
Kiuchi, Hiroshi;Tsujimura, Akira;Okuyama, Akihiko
通讯作者：
Okuyama, Akihiko
影响因子：
2.1
作者：
Lee, Jane-Dar;Lee, Ming-Huei
通讯作者：
Lee, Ming-Huei
影响因子：
6.6
作者：
Hodges, Steve J.;Zhou, Ge;Christ, George J.
通讯作者：
Christ, George J.
影响因子：
4.5
作者：
Dagher A;Curatolo A;Sachdev M;Stephens AJ;Mullins C;Landis JR;van Bokhoven A;El-Hayek A;Froehlich JW;Briscoe AC;Roy R;Yang J;Pontari MA;Zurakowski D;Lee RS;Moses MA;MAPP Research Network
通讯作者：
MAPP Research Network
影响因子：
6.6
作者：
IRWIN, P;GALLOWAY, NTM
通讯作者：
GALLOWAY, NTM