Organoids capture tissue-specific innate lymphoid cell development in mice and humans.
Organoids capture tissue-specific innate lymphoid cell development in mice and humans.
复制标题
类器官捕捉小鼠和人类中组织特异性先天淋巴细胞的发育。
DOI：
10.1016/j.celrep.2022.111281
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发表时间：
2022-08-30
期刊：
影响因子：
8.8
通讯作者：
Neves, Joana F.
中科院分区：
文献类型：
--
作者：
Jowett, Geraldine M.;Read, Emily;Roberts, Luke B.;Coman, Diana;Gonzalez, Marta Vila;Zabinski, Tomasz;Niazi, Umar;Reis, Rita;Trieu, Tung-Jui;Danovi, Davide;Gentleman, Eileen;Vallier, Ludovic;Curtis, Michael A.;Lord, Graham M.;Neves, Joana F.
介绍了基于器官的小鼠和人先天淋巴细胞前体细胞(ILCP)成熟模型。首先，利用小鼠的肠道和肺器官来证明，上皮龛足以并行地驱动所有固有淋巴样细胞(ILC)组的组织特异性成熟，而不需要亚群特异性细胞因子的补充。然后，使用更复杂的基于人类诱导多能干细胞(HiPSC)的肠道和肺器官模型来证明，人上皮细胞从严格的全身性人类iLCP群体中重现ILC的成熟，但仅当与器官相关的基质细胞耗尽时。这些系统提供了多才多艺的简化论者模型来剖析环境和粘膜生态位线索对ILC成熟的影响。在未来，这些可能为了解ILC的活动和发育如何在慢性炎症性疾病中变得失调提供了洞察力。有机化合物强有力地捕获原位小鼠和人类ILC发育ILC成熟的起源诱导持久的组织特异性印记人类肠道上皮，而不是基质，支持人类iLCP的增殖和成熟。表明肠道和肺器官支持所有固有淋巴样细胞(ILCs)亚群的产生。有机物来源的ILCs的表型和功能与活体对应的ILCs相匹配。因此，上皮生态位对ILC的分化至关重要，并足以赋予组织特异性特性。
Organoid-based models of murine and human innate lymphoid cell precursor (ILCP) maturation are presented. First, murine intestinal and pulmonary organoids are harnessed to demonstrate that the epithelial niche is sufficient to drive tissue-specific maturation of all innate lymphoid cell (ILC) groups in parallel, without requiring subset-specific cytokine supplementation. Then, more complex human induced pluripotent stem cell (hiPSC)-based gut and lung organoid models are used to demonstrate that human epithelial cells recapitulate maturation of ILC from a stringent systemic human ILCP population, but only when the organoid-associated stromal cells are depleted. These systems offer versatile and reductionist models to dissect the impact of environmental and mucosal niche cues on ILC maturation. In the future, these could provide insight into how ILC activity and development might become dysregulated in chronic inflammatory diseases. Organoids robustly capture in situ murine and human ILC development Origin of ILC maturation induces lasting tissue-specific imprint Human gut epithelium, not stroma, supports proliferation and maturation of human ILCP Jowett et al. show that intestinal and lung organoids support the generation of all innate lymphoid cells (ILCs) subsets. The phenotypes and functions of organoid-derived ILCs match in vivo counterparts. The epithelial niche is thus critical for the differentiation of ILCs and is sufficient to confer tissue-specific properties.
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影响因子：
16.6
作者：
Jung KB;Lee H;Son YS;Lee MO;Kim YD;Oh SJ;Kwon O;Cho S;Cho HS;Kim DS;Oh JH;Zilbauer M;Min JK;Jung CR;Kim J;Son MY
通讯作者：
Son MY
影响因子：
5.4
作者：
KIESSLING, R;KLEIN, E;WIGZELL, H
通讯作者：
WIGZELL, H
影响因子：
30.5
作者：
Bernink, Jochem H.;Ohne, Yoichiro;Humbles, Alison A.
通讯作者：
Humbles, Alison A.
DOI：
10.1007/978-1-4939-6786-5_17
发表时间：
2017-01-01
期刊：
INFLAMMATION: METHODS AND PROTOCOLS
影响因子：
--
作者：
Gronke, Konrad;Kofoed-Nielsen, Michael;Diefenbach, Andreas
通讯作者：
Diefenbach, Andreas
影响因子：
30.5
作者：
Bernink, Jochem H.;Peters, Charlotte P.;Spits, Hergen
通讯作者：
Spits, Hergen