Transcriptomic and Metabolomic Responses of Rice Plants to Cnaphalocrocis medinalis Caterpillar Infestation.
Transcriptomic and Metabolomic Responses of Rice Plants to Cnaphalocrocis medinalis Caterpillar Infestation.
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水稻植株对稻纵卷叶螟毛虫侵染的转录组学和代谢组学反应
DOI：
10.3390/insects11100705
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发表时间：
2020-10-15
期刊：
影响因子：
3
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Wang Y;Liu Q;Du L;Hallerman EM;Li Y
为了更好地了解水稻对稻纵卷叶螟的防御机制，研究了稻纵卷叶螟侵染后水稻叶片中转录组和代谢组的差异。结果表明，C.稻纵卷叶螟（medinalis）侵染可诱导水稻叶片发生快速、精确的防御反应，而茉莉酸（jasmonic acid，JA）依赖的信号通路在水稻对该害虫的反应中起着重要作用。这些结果为深入了解水稻对稻纵卷叶螟的防御系统提供了全面的信息，并可能有助于通过确定分子靶标进行选择来开发抗虫水稻品种。摘要植物与植食性昆虫之间的相互作用是决定农田和自然农田植物生产力的重要因素。稻纵卷叶螟（Cnaphalocrocis medinalis）对亚洲国家的水稻生产造成了巨大的危害。然而，关于水稻植物如何在分子和生物化学水平上防御这种破坏性害虫的信息很少。本研究以水稻叶片为材料，观察了C. medinalis使用RNA测序和代谢组学分析。转录分析表明，基因表达迅速响应叶夹侵扰，最显着的转录变化发生在6小时内开始喂养后。代谢产物丰度的变化比基因表达的变化慢。基因本体论和京都基因百科全书和基因组富集分析表明，水稻对虫害的转录反应涉及编码蛋白激酶、转录因子、次生代谢物生物合成、光合作用和植物激素信号的基因。此外，茉莉酸依赖的信号通路触发的叶蝉草食性在水稻防御该害虫发挥了至关重要的作用。总之，我们的研究结果提供了全面的见解水稻的防御系统，这一物种，并可能告知抗虫水稻品种的发展。
Simple Summary The transcriptomic and metabolomic differences in rice leaves after infestation by the rice leaf folder Cnaphalocrocis medinalis were investigated for better understanding of the mechanisms of rice defenses against this species. The results suggest that C. medinalis infestation can induce rapid and precise defense responses involved in many primary and secondary metabolic processes in rice leaves, and the jasmonic acid (JA)-dependent signaling pathway plays vital roles in the response of rice plants to this pest species. These results provide comprehensive insights into the defense system of rice to the rice leaf folder and may facilitate the development of insect-resistant rice varieties by identifying molecular targets for selection. Abstract Interactions between plants and insect herbivores are important determinants of plant productivity in cultivated and natural agricultural fields. The rice leaf folder (Cnaphalocrocis medinalis) causes tremendous damage to rice production in Asian countries. However, little information is available about how rice plants defend themselves against this destructive pest at molecular and biochemical levels. Here, we observed the transcriptomic and metabolomic differences in rice leaves after 0, 1, 6, 12, and 24 h of being fed by C. medinalis using RNA sequencing and metabolome profiling. Transcriptional analyses showed that gene expression responds rapidly to leaf folder infestation, with the most significant transcriptional changes occurring within 6 h after the initiation of feeding. Metabolite abundance changed more slowly than gene expression. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses indicated that the rice transcriptional response to infestation involved genes encoding protein kinases, transcription factors, biosynthesis of secondary metabolites, photosynthesis, and phytohormone signaling. Moreover, the jasmonic acid-dependent signaling pathway triggered by leaf folder herbivory played a vital role in rice defense against this pest. Taken together, our results provide comprehensive insights into the defense system of rice to this species and may inform the development of insect-resistant rice varieties.
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影响因子：
5.6
作者：
Liu Q;Hallerman E;Peng Y;Li Y
通讯作者：
Li Y
影响因子：
5.6
作者：
Chintalapati, Padmavathi;Balakrishnan, Divya;Katti, Gururaj
通讯作者：
Katti, Gururaj
影响因子：
3.7
作者：
Heyer M;Scholz SS;Voigt D;Reichelt M;Aldon D;Oelmüller R;Boland W;Mithöfer A
通讯作者：
Mithöfer A
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影响因子：
27.5
作者：
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通讯作者：
Luo, Jie
影响因子：
2.3
作者：
Lou, YG;Du, MH;Shan, WF
通讯作者：
Shan, WF