Genome-Wide Identification and Expression Profiling of the WOX Gene Family in Citrus sinensis and Functional Analysis of a CsWUS Member.
Genome-Wide Identification and Expression Profiling of the WOX Gene Family in Citrus sinensis and Functional Analysis of a CsWUS Member.
复制标题
DOI：
10.3390/ijms22094919
复制
发表时间：
2021-05-06
影响因子：
5.6
通讯作者：
Hu CG
中科院分区：
文献类型：
--
作者：
Shafique Khan F;Zeng RF;Gan ZM;Zhang JZ;Hu CG
WUSCHEL相关同源异型盒（WOX）转录因子（TF）在植物发育中的作用是众所周知的，但很少在柑橘中进行研究。在这项研究中，我们确定了11个推定的基因从甜橙子基因组和柑橘WOX基因分为三个分支（现代/WUSCHEL（WUS），中间，和古代）。随后，我们进行了同线关系，内含子-外显子组织，模体组成，顺式元件分析。基于RNA-seq和组织特异性表达模式的共表达分析揭示CsWOX基因表达具有多种内在功能。AtWUS的CsWUS同源物作为转录激活因子发挥功能并结合特异性DNA。CsWUS在烟草中的过表达揭示了戏剧性的表型变化，包括畸形叶和减少雌蕊花没有种子发育。使用病毒诱导的基因沉默（VIGS）系统沉默柠檬中的CsWUS意味着CsWUS参与植物茎细胞。此外，发现CsWUS与拟南芥中的直系同源物CsCYCD 3（AtCYCD 3，1）相互作用。酵母单杂交筛选和双荧光素酶活性检测表明，CsRAP 2.12和CsHB 22两个转录因子与CsWUS的启动子结合并调控其表达。这些结果丰富了我们对WOX基因家族沿着CsWUS功能的认识，为进一步研究WOX基因家族成员在柑橘中的发育调控和综合数据提供了有价值的发现。
WUSCHEL-related homeobox (WOX) transcription factors (TFs) are well known for their role in plant development but are rarely studied in citrus. In this study, we identified 11 putative genes from the sweet orange genome and divided the citrus WOX genes into three clades (modern/WUSCHEL(WUS), intermediate, and ancient). Subsequently, we performed syntenic relationship, intron-exon organization, motif composition, and cis-element analysis. Co-expression analysis based on RNA-seq and tissue-specific expression patterns revealed that CsWOX gene expression has multiple intrinsic functions. CsWUS homolog of AtWUS functions as a transcriptional activator and binds to specific DNA. Overexpression of CsWUS in tobacco revealed dramatic phenotypic changes, including malformed leaves and reduced gynoecia with no seed development. Silencing of CsWUS in lemon using the virus-induced gene silencing (VIGS) system implied the involvement of CsWUS in cells of the plant stem. In addition, CsWUS was found to interact with CsCYCD3, an ortholog in Arabidopsis (AtCYCD3,1). Yeast one-hybrid screening and dual luciferase activity revealed that two TFs (CsRAP2.12 and CsHB22) bind to the promoter of CsWUS and regulate its expression. Altogether, these results extend our knowledge of the WOX gene family along with CsWUS function and provide valuable findings for future study on development regulation and comprehensive data of WOX members in citrus.
登录
查看更多内容
影响因子：
5.6
作者：
Ahmad, Bilal;Zhang, Songlin;Wang, Xiping
通讯作者：
Wang, Xiping
影响因子：
3
作者：
Arroyo-Herrera, A.;Gonzalez, A. Ku;Castano, E.
通讯作者：
Castano, E.
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS
空间生长素信号控制拟南芥叶片扁平化。
DOI：
10.1016/j.cub.2017.08.042
发表时间：
2017-10-09
期刊：
Current biology : CB
影响因子：
--
作者：
Guan C;Wu B;Yu T;Wang Q;Krogan NT;Liu X;Jiao Y
通讯作者：
Jiao Y
影响因子：
--
作者：
Holland, Peter W. H.
通讯作者：
Holland, Peter W. H.