Synthesis, Characterization and in Vitro Evaluation of Manganese Ferrite (MnFe2O4) Nanoparticles for Their Biocompatibility with Murine Breast Cancer Cells (4T1).
Synthesis, Characterization and in Vitro Evaluation of Manganese Ferrite (MnFe2O4) Nanoparticles for Their Biocompatibility with Murine Breast Cancer Cells (4T1).
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DOI：
10.3390/molecules21030312
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发表时间：
2016-03-11
期刊：
影响因子：
--
通讯作者：
Purna Chandra Rao B
中科院分区：
文献类型：
--
作者：
Kanagesan S;Aziz SB;Hashim M;Ismail I;Tamilselvan S;Alitheen NB;Swamy MK;Purna Chandra Rao B
以硝酸铁和硝酸锰为原料，通过溶胶-凝胶自燃烧技术成功制备了铁酸锰（MnFe2O4）磁性纳米颗粒，并在150℃下煅烧24小时。通过 X 射线衍射 (XRD)、傅里叶变换红外光谱 (FTIR)、透射电子显微镜 (TEM) 和振动样品磁强计 (VSM) 对煅烧样品进行系统表征，以确定晶相、官能团、形态、粒径、形状和磁性行为。结果表明，在低温下获得的脊柱铁氧体表现出单相、纳米颗粒尺寸和良好的磁性能。研究结果表明，不同浓度的MnFe2O4纳米粒子对4T1细胞系存在有效的剂量依赖性细胞毒作用，孵育24小时、48小时和72小时后IC50值分别为210、198和171 μg/mL。暴露于较高浓度纳米粒子的细胞显示出细胞凋亡和坏死活性逐渐增加。浓度低于 125 μg/mL 时，纳米颗粒与 4T1 细胞具有生物相容性。
Manganese ferrite (MnFe2O4) magnetic nanoparticles were successfully prepared by a sol-gel self-combustion technique using iron nitrate and manganese nitrate, followed by calcination at 150 °C for 24 h. Calcined sample was systematically characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and vibrational sample magnetometry (VSM) in order to identify the crystalline phase, functional group, morphology, particle size, shape and magnetic behavior. It was observed that the resultant spinal ferrites obtained at low temperature exhibit single phase, nanoparticle size and good magnetic behavior. The study results have revealed the existence of a potent dose dependent cytotoxic effect of MnFe2O4 nanoparticles against 4T1 cell lines at varying concentrations with IC50 values of 210, 198 and 171 μg/mL after 24 h, 48 h and 72 h of incubation, respectively. Cells exposed to higher concentrations of nanoparticles showed a progressive increase of apoptotic and necrotic activity. Below 125 μg/mL concentration the nanoparticles were biocompatible with 4T1 cells.
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影响因子：
14
作者：
Cui, Xianjin;Belo, Salome;Krueger, Dirk;Yan, Yong;de Rosales, Rafael T. M.;Jauregui-Osoro, Maite;Ye, Haitao;Su, Shi;Mathe, Domokos;Kovacs, Noemi;Horvath, Ildiko;Semjeni, Mariann;Sunassee, Kavitha;Szigeti, Krisztian;Green, Mark A.;Blower, Philip J.
通讯作者：
Blower, Philip J.
影响因子：
2.1
作者：
Krishnan KM
通讯作者：
Krishnan KM
影响因子：
--
作者：
Kanagesan, S.;Hashim, M.;Ahsanul, K.
通讯作者：
Ahsanul, K.
影响因子：
4.6
作者：
Deng, Hong;Chen, Hongyu;Li, He
通讯作者：
Li, He
影响因子：
2.7
作者：
Lakshman, A;Rao, KH;Mendiratta, RG
通讯作者：
Mendiratta, RG