Bacterial distribution on the ocular surface of patients with primary Sjögren's syndrome.
Bacterial distribution on the ocular surface of patients with primary Sjögren's syndrome.
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DOI：
10.1038/s41598-022-05625-w
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发表时间：
2022-02-02
影响因子：
4.6
通讯作者：
Hwang HB
中科院分区：
文献类型：
--
作者：
Kim YC;Ham B;Kang KD;Yun JM;Kwon MJ;Kim HS;Hwang HB
许多研究表明，肠道微生物失调是自身免疫性疾病病因学的一个主要因素，但没有一项研究表明眼表 (OS) 微生物群与干燥综合征 (SS) 相关。在这项前瞻性研究中，我们分析了原发性 SS 患者 OS 中的细菌分布。在本研究纳入的 120 名受试者中，48 名患者（A 组）患有原发性 SS，而 72 名受试者（B 组）患有与 SS 无关的干眼症状。我们评估了临床干眼参数，例如 OS 疾病指数、眼染色评分 (OSS)、Schirmer’s I 测试和泪膜破裂时间 (TBUT)。使用结膜拭子分析两组的微生物群落。使用Illumina MiSeq平台对细菌16S rRNA基因进行测序，并使用QIIME 1.9.1程序对数据进行分析。 A 组微生物群的香农指数显着低于 B 组微生物群（p<<0.05）。使用布雷-柯蒂斯距离法进行相似性分析发现两组之间的β多样性没有差异（p > 0.05）。 A组中门水平的放线菌和属水平的棒状杆菌丰度低于B组，但差异无统计学意义（p>0.05）。 SS 明显降低了 OS 微生物群落的多样性。这些观察结果可能与 SS 的病理生理学有关，应在未来的研究中进行调查。
Many studies have shown that gut microbial dysbiosis is a major factor in the etiology of autoimmune diseases but none have suggested that the ocular surface (OS) microbiome is associated with Sjögren’s syndrome (SS). In this prospective study, we analyzed bacterial distribution on the OS in patients with primary SS. Among the 120 subjects included in this study, 48 patients (group A) had primary SS, whereas 72 subjects (group B) had dry eye symptoms that were unrelated to SS. We evaluated clinical dry eye parameters such as the OS disease index, ocular staining score (OSS), Schirmer’s I test, and tear break-up time (TBUT). Conjunctival swabs were used to analyze the microbial communities from the two groups. Bacterial 16S rRNA genes were sequenced using the Illumina MiSeq platform, and the data were analyzed using the QIIME 1.9.1 program. The Shannon index was significantly lower in group A than in group B microbiota (p < 0.05). An analysis of similarity using the Bray–Curtis distance method found no difference in beta-diversity between the two groups (p > 0.05). In group A, Actinobacteria at the phylum level and Corynebacteria at the genus level exhibited low abundance than group B, but the differences were not statistically significant (p > 0.05). SS apparently decreases the diversity of the OS microbial community. These observations may be related to the pathophysiology of SS and should be investigated in future studies.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1073/pnas.1205624109
发表时间：
2012-05-22
影响因子：
11.1
作者：
Hanski, Ilkka;von Hertzen, Leena;Haahtela, Tari
通讯作者：
Haahtela, Tari
影响因子：
2
作者：
Ham, Baknoon;Hwang, Hyung Bin;Kwon, Man Jae
通讯作者：
Kwon, Man Jae
影响因子：
4.4
作者：
Lee, Se Hee;Oh, Doo Hwan;Jeon, Che Ok
通讯作者：
Jeon, Che Ok
影响因子：
24.5
作者：
Cani PD;Possemiers S;Van de Wiele T;Guiot Y;Everard A;Rottier O;Geurts L;Naslain D;Neyrinck A;Lambert DM;Muccioli GG;Delzenne NM
通讯作者：
Delzenne NM