Association between the luteinizing hormone/chorionic gonadotropin receptor (LHCGR) rs4073366 polymorphism and ovarian hyperstimulation syndrome during controlled ovarian hyperstimulation.
Association between the luteinizing hormone/chorionic gonadotropin receptor (LHCGR) rs4073366 polymorphism and ovarian hyperstimulation syndrome during controlled ovarian hyperstimulation.
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DOI：
10.1186/1477-7827-11-71
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发表时间：
2013-07-25
期刊：
影响因子：
--
通讯作者：
Gindoff P
中科院分区：
文献类型：
--
作者：
O'Brien TJ;Kalmin MM;Harralson AF;Clark AM;Gindoff I;Simmens SJ;Frankfurter D;Gindoff P
本研究旨在探讨促黄体生成素/绒毛膜促性腺激素(LHCGR)高功能基因(rs4539842/insLQ)与控制性超排卵(COH)结局的关系。这是一项前瞻性研究，研究对象为GWU生育与试管受精中心的172名COH患者。从血液样本中提取DNA，并对包含insLQ多态的区域进行测序。我们还研究了Ins LQ的一个142bp的多态性(rs4073366 G >； C)。分析insLQ和rs4073366等位基因与COH(成熟卵泡数、人绒毛膜促性腺激素(HCG)注射日雌二醇水平、取卵数和卵巢过度刺激综合征(OHSS))的相关性。年龄增长和第3天(基础)FSH水平较高与COH反应较差显著相关。我们发现insLQ和rs4073366都处于连锁不平衡(LD)状态，没有患者是两个隐性等位基因(insLQ/insLQ；C/C)的纯合子。Ins LQ变异与COH的任何主要结局均无显著关联。Rs4073366C变异的携带者状态(P = 0.033)与发生OHS的风险增加(OR2.95，95%CI = 1.09-7.96)相关。虽然年龄和第3天的FSH水平可以预测预后，但我们发现Ins LQ和患者对COH的反应之间没有关联。有趣的是，rs4073366 C变异携带者状态与OHSS风险相关。据我们所知，这是第一份表明LHCGR基因变异可能在OHSS患者风险中发挥作用的报告。
The aim of this study was to determine the relationship between a purported luteinizing hormone/chorionic gonadotropin (LHCGR) high function polymorphism (rs4539842/insLQ) and outcome to controlled ovarian hyperstimulation (COH). This was a prospective study of 172 patients undergoing COH at the Fertility and IVF Center at GWU. DNA was isolated from blood samples and a region encompassing the insLQ polymorphism was sequenced. We also investigated a polymorphism (rs4073366 G > C) that was 142 bp from insLQ. The association of the insLQ and rs4073366 alleles and outcome to COH (number of mature follicles, estradiol level on day of human chorionic gonadotropin (hCG) administration, the number of eggs retrieved and ovarian hyperstimulation syndrome (OHSS)) was determined. Increasing age and higher day 3 (basal) FSH levels were significantly associated with poorer response to COH. We found that both insLQ and rs4073366 were in linkage disequilibrium (LD) and no patients were homozygous for both recessive alleles (insLQ/insLQ; C/C). The insLQ variant was not significantly associated with any of the main outcomes to COH. Carrier status for the rs4073366 C variant was associated (P = 0.033) with an increased risk (OR 2.95, 95% CI = 1.09-7.96) of developing OHSS. While age and day 3 FSH levels were predictive of outcome, we found no association between insLQ and patient response to COH. Interestingly, rs4073366 C variant carrier status was associated with OHSS risk. To the best of our knowledge, this is the first report suggesting that LHCGR genetic variation might function in patient risk for OHSS.
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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