Genetic and physiological regulation of folate in pak choi (Brassica rapa subsp. Chinensis) germplasm
Genetic and physiological regulation of folate in pak choi (Brassica rapa subsp. Chinensis) germplasm
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小白菜种质中叶酸的遗传和生理调控
DOI：
10.1093/jxb/eraa218
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发表时间：
2020-07
影响因子：
6.9
通讯作者：
Yang Xiaoe
中科院分区：
文献类型：
--
作者：
Shohag M. J. I.;Wei Yanyan;Zhang Jie;Feng Ying;Rychlik Michael;He Zhenli;Yang Xiaoe
叶酸是所有生物体必需的微量营养素之一。由于饮食摄入不足，叶酸缺乏症在人类中仍然普遍存在。遗传多样性种质可用作育种计划中的亲本，也可用于了解叶酸调控网络。因此，我们研究了小白菜（Brassica rapa subsp. Chinensis）种质中叶酸的天然遗传多样性及其生理调控。总叶酸浓度范围为52.7μg 100gFW-1至166.9μg 100gFW(-1)，变化3.2倍。主要的叶酸维生素体以5-CH3-H(4)叶酸为代表，有4.5倍的变异。 GTP环水解酶I和氨基脱氧分支酸合酶（叶酸合成的第一步）的活性在高叶酸材料中较高，而在低叶酸材料中较低。对与叶酸代谢相关的11个基因的转录水平的分析表明，叶酸浓度的差异可能主要控制在转录后水平。观察到总叶酸与其前体之间的一般相关性。叶酸多样性和叶绿素含量通过甲基循环受到严格调节。小白菜种质的多样化遗传变异表明，整合叶酸生物强化育种计划和揭示植物叶酸稳态的生理基础具有巨大的遗传潜力。
Folates are one of the essential micronutrients for all living organisms. Due to inadequate dietary intake, folate deficiency remains prevalent in humans. Genetically diverse germplasms can potentially be used as parents in breeding programs and also for understanding the folate regulatory network. Therefore, we investigated the natural genetic diversity of folates and their physiological regulation in pak choi (Brassica rapa subsp. Chinensis) germplasm. The total folate concentration ranged from 52.7 mu g 100 gFW-1 to 166.9 mu g 100 gFW(-1), with 3.2-fold variation. The main folate vitamer was represented by 5-CH3-H(4)folate, with 4.5-fold variation. The activities of GTP cyclohydrolase I and aminodeoxy chorismate synthase, the first step of folate synthesis, were high in high folate accessions and low in low folate accessions. Analysis of the transcription levels of 11 genes associated with folate metabolism demonstrated that the difference in folate concentrations may be primarily controlled at the post-transcriptional level. A general correlation between total folate and their precursors was observed. Folate diversity and chlorophyll content were tightly regulated through the methyl cycle. The diverse genetic variation in pak choi germplasm indicated the great genetic potential to integrate breeding programs for folate biofortification and unravel the physiological basis of folate homeostasis in planta.
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影响因子：
46.9
作者：
K. Ramessar;T. Capell;R. Twyman;H. Quemada;P. Christou
通讯作者：
K. Ramessar;T. Capell;R. Twyman;H. Quemada;P. Christou
DOI：
10.3390/agronomy9110734
发表时间：
2019-11
期刊：
Agronomy
影响因子：
--
作者：
B. R. Robinson;Carolina García Salinas;Perla Ramos Parra;J. Bamberg;R. I. Díaz de la Garza;A. Goyer
通讯作者：
B. R. Robinson;Carolina García Salinas;Perla Ramos Parra;J. Bamberg;R. I. Díaz de la Garza;A. Goyer
DOI：
10.1073/pnas.0700640104
发表时间：
2007-03
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
D. DellaPenna
通讯作者：
D. DellaPenna
影响因子：
7.4
作者：
Karen Loizeau;B. Gambonnet;Guo-Fang Zhang;G. Curien;Samuel Jabrin;D. Van Der Straeten;W. Lambert;F. Rébeillé;S. Ravanel
通讯作者：
Karen Loizeau;B. Gambonnet;Guo-Fang Zhang;G. Curien;Samuel Jabrin;D. Van Der Straeten;W. Lambert;F. Rébeillé;S. Ravanel
影响因子：
4
作者：
Moore, Eileen M.;Ames, David;Watters, David A.
通讯作者：
Watters, David A.