Role of the Aryl Hydrocarbon Receptor in the Pathogenesis of Chronic Rhinosinusitis with Nasal Polyps
Role of the Aryl Hydrocarbon Receptor in the Pathogenesis of Chronic Rhinosinusitis with Nasal Polyps
复制标题
芳基烃受体在慢性鼻窦炎伴鼻息肉发病机制中的作用
DOI：
10.1007/s10753-013-9751-7
复制
发表时间：
2014-04
期刊：
影响因子：
5.1
通讯作者：
Hong, Su-ling
中科院分区：
文献类型：
--
作者：
Yao, Hong-bing;Kou, Wei;Zhang, Cheng;Hong, Su-ling
在中国患者中，主要的Th 17群体是慢性鼻窦炎伴鼻息肉（CRSwNP）的标志物。芳烃受体（arylhydrocarbonreceptor，AhR）作为一种配体激活的转录因子，在促进或抑制特异性Th细胞发育中起着重要作用。然而，它在CRSwNP中的作用仍有待确定。本研究的目的是调查是否AhR，调节Th 17细胞分化，发挥了作用，CRSwNP的发病机制，通过评估AhR表达鼻息肉和外周血单核细胞（PBMC）从CRSwNP患者。48例患者（特应性，24;非特应性，24）和13名对照进行了研究。为了探讨AhR在CRSwNP中的作用，我们使用mRNA或蛋白检测方法分析AhR、维甲酸相关孤儿受体C（RORC）、白细胞介素（IL）-17和IL-10的表达以及Th 17的分化。值得注意的是，CRSwNP中AhR的表达降低，并且特应性组中AhR的表达低于非特应性组。然而，与两个CRSwNP组相比，对照组中Th 17及其相关因子（RORC，IL-17）的水平非常低。特别是，特应性CRSwNP患者的Th 17细胞的极化与非特应性个体相比增加。此外，ITE干预PBMC促进AhR表达并减弱Th 17应答，表明AhR更可能抑制中国CRSwNP患者的Th 17细胞分化。这一信息是有价值的，以获得一个清晰的了解CRSwNP的发病机制。此外，特应性CRSwNP患者可能表现出AhR表达减少，导致息肉组织和PBMC中Th 17细胞的不成比例分布加剧，从而表明特应性CRSwNP具有与非特应性CRSwNP不同的发病机制。
A predominant Th17 population is a marker of chronic rhinosinusitis with nasal polyps (CRSwNP) in Chinese patients. As a ligand-activated transcription factor, the aryl hydrocarbon receptor (AhR) plays a vital role in promoting or inhibiting specific Th cell development. However, its role in CRSwNP remains to be defined. The aim of the present study was to investigate whether AhR, which regulates Th17 cell differentiation, played a role in the pathogenesis of CRSwNP by evaluating AhR expression in nasal polyps and peripheral blood mononuclear cells (PBMCs) obtained from CRSwNP patients. Forty-eight patients (atopic, 24; non-atopic, 24) and 13 controls were studied. To explore the role of AhR in CRSwNP, we analyzed the expression of AhR, retinoid-related orphan receptor C (RORC), interleukin (IL)-17, and IL-10 and the differentiation of Th17 using mRNA or protein detection methods. Notably, the expression of AhR was reduced in CRSwNP, and the expression of AhR was lower in the atopic group than in the non-atopic group. However, there was a very low level of Th17 and its associated factors (RORC, IL-17) in the control group compared to the two CRSwNP groups. In particular, the polarization of Th17 cells in atopic CRSwNP patients was increased compared with non-atopic individuals. In addition, ITE intervention in PBMCs promoted AhR expression and attenuated Th17 responses, demonstrating that AhR was more likely to suppress Th17 cells differentiation in Chinese CRSwNP patients. This information is valuable for obtaining a clear understanding of the pathogenesis of CRSwNP. Moreover, patients with atopic CRSwNP may exhibit reduced expression of AhR, leading to aggravation of the disproportionate distribution of Th17 cells in polyp tissues and PBMCs, thereby suggesting that atopic CRSwNP has a distinct pathogenesis from that of non-atopic CRSwNP.
登录
查看更多内容
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
7.2
作者：
Fokkens, Wytske J.;Lund, Valerie J.;Wormald, Peter John
通讯作者：
Wormald, Peter John
影响因子：
5
作者：
Jing Zhu;Yong Cao;Kai-yan Li;Zhengyun Wang;P. Zuo;W. Xiong;Yong-jian Xu;S. Xiong
通讯作者：
Jing Zhu;Yong Cao;Kai-yan Li;Zhengyun Wang;P. Zuo;W. Xiong;Yong-jian Xu;S. Xiong
影响因子：
32.4
作者：
Stark, MA;Huo, YQ;Ley, K
通讯作者：
Ley, K
影响因子：
3.7
作者：
Shen, Y.;Tang, X. -Y.;Hong, S. -L.
通讯作者：
Hong, S. -L.