The effects of glucosinolates and their breakdown products on necrotrophic fungi.
The effects of glucosinolates and their breakdown products on necrotrophic fungi.
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DOI：
10.1371/journal.pone.0070771
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Levy M
中科院分区：
文献类型：
--
作者：
Buxdorf K;Yaffe H;Barda O;Levy M
硫代葡萄糖苷是一类含硫和含氮的次生代谢产物，在植物防御中起着多种作用。在这项研究中，我们使用拟南芥突变体，含有不同量的芥子油苷和芥子油苷分解产物，研究这些植物化学物质对植物病原真菌的影响。我们比较了真菌灰葡萄孢，感染各种主机，与拟南芥科特有的真菌芸苔链格孢。B。灰葡萄孢菌株对硫代葡萄糖苷及其水解产物的敏感性依赖于不同的组分，而A.芸苔属植物对脂肪族芥子油苷和异硫氰酸酯的分解产物影响更大。我们还发现，B.灰葡萄对硫代葡萄糖苷积累的刺激程度大于A.芸苔属。在我们的工作与A。对于芸苔属，我们发现芥子油苷分解产物的类型比衍生该产物的芥子油苷的类型更重要，如Ler背景的敏感性和表达表硫指定蛋白的Col-0植物中获得的敏感性所证明的，这两种蛋白都积累简单腈和表硫腈，但不积累异硫氰酸酯。此外，在体内，吲哚芥子油苷的水解产物被发现参与防御B。cinerea，但不参与寄主对A.芸苔属。我们建议将菊科植物A.芸苔属已经适应了吲哚类硫代葡萄糖酸的存在，并能够应对其水解产物。与此相反，一些分离的通才B。灰葡萄对这些植物化学物质更敏感。
Glucosinolates are a diverse class of S- and N-containing secondary metabolites that play a variety of roles in plant defense. In this study, we used Arabidopsis thaliana mutants that contain different amounts of glucosinolates and glucosinolate-breakdown products to study the effects of these phytochemicals on phytopathogenic fungi. We compared the fungus Botrytis cinerea, which infects a variety of hosts, with the Brassicaceae-specific fungus Alternaria brassicicola. B. cinerea isolates showed variable composition-dependent sensitivity to glucosinolates and their hydrolysis products, while A. brassicicola was more strongly affected by aliphatic glucosinolates and isothiocyanates as decomposition products. We also found that B. cinerea stimulates the accumulation of glucosinolates to a greater extent than A. brassicicola. In our work with A. brassicicola, we found that the type of glucosinolate-breakdown product is more important than the type of glucosinolate from which that product was derived, as demonstrated by the sensitivity of the Ler background and the sensitivity gained in Col-0 plants expressing epithiospecifier protein both of which accumulate simple nitrile and epithionitriles, but not isothiocyanates. Furthermore, in vivo, hydrolysis products of indole glucosinolates were found to be involved in defense against B. cinerea, but not in the host response to A. brassicicola. We suggest that the Brassicaceae-specialist A. brassicicola has adapted to the presence of indolic glucosinolates and can cope with their hydrolysis products. In contrast, some isolates of the generalist B. cinerea are more sensitive to these phytochemicals.
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影响因子：
9.4
作者：
Gigolashvili, Tamara;Engqvist, Martin;Fluegge, Ulf-Ingo
通讯作者：
Fluegge, Ulf-Ingo
影响因子：
7.4
作者：
Celenza, JL;Quiel, JA;Bender, J
通讯作者：
Bender, J
影响因子：
11.6
作者：
Boettcher, Christoph;Westphal, Lore;Glawischnig, Erich
通讯作者：
Glawischnig, Erich
DOI：
10.1073/pnas.021551098
发表时间：
2001-01-16
影响因子：
11.1
作者：
Hagemeier, J;Schneider, B;Hahlbrock, K
通讯作者：
Hahlbrock, K
影响因子：
7.4
作者：
Brader, G;Tas, É;Palva, ET
通讯作者：
Palva, ET