Protein methylation in full length Chlamydomonas flagella.
Protein methylation in full length Chlamydomonas flagella.
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DOI：
10.1002/cm.20387
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发表时间：
2009-08
影响因子：
--
通讯作者：
Howard, Louisa
中科院分区：
文献类型：
--
作者：
Sloboda, Roger D.;Howard, Louisa
翻译后蛋白修饰广泛发生在真核鞭毛中。在这里，我们研究蛋白质甲基化，一种蛋白质修饰，最近才报道发生在鞭毛。蛋氨酸合成酶（MetE）的钴胺素（维生素B12）独立形式，催化蛋氨酸生产的最后一步，定位于鞭毛。在这里，我们证明，使用免疫金扫描电子显微镜，MetE是结合到鞭毛的外部双峰。蛋氨酸可以转化为s -腺苷型蛋氨酸，然后作为蛋白质甲基化反应的甲基供体。利用识别对称或不对称甲基化精氨酸残基的抗体，我们在完整鞭毛中鉴定出三种高度甲基化的蛋白：两个约30和40 kDa的对称甲基化蛋白，以及一个约75 kDa的不对称甲基化蛋白。其他几个相对较少甲基化的蛋白质也可以被检测到。分离和免疫印迹分析表明，这些蛋白是鞭毛轴突素的组成部分。免疫金薄片电镜显示，对称甲基化蛋白位于轴突的中心区域，可能是中心对复合体和径向辐条的组成部分，而不对称甲基化蛋白与外部双线有关。
Post-translational protein modification occurs extensively in eukaryotic flagella. Here we examine protein methylation, a protein modification that has only recently been reported to occur in flagella. The cobalamin (vitamin B12) independent form of the enzyme methionine synthase (MetE), which catalyzes the final step in methionine production, is localized to flagella. Here we demonstrate, using immunogold scanning electron microscopy, that MetE is bound to the outer doublets of the flagellum. Methionine can be converted to S-adenosyl methionine, which then serves as the methyl donor for protein methylation reactions. Using antibodies that recognize symmetrically or asymmetrically methylated arginine residues, we identify three highly methylated proteins in intact flagella: two symmetrically methylated proteins of about 30 and 40 kDa, and one asymmetrically methylated protein of about 75 kDa. Several other relatively less methylated proteins could also be detected. Fractionation and immunoblot analysis shows that these proteins are components of the flagellar axoneme. Immunogold thin section electron microscopy indicates that the symmetrically methylated proteins are located in the central region of the axoneme, perhaps as components of the central pair complex and the radial spokes, while the asymmetrically methylated proteins are associated with the outer doublets.
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影响因子：
4
作者：
Bedford, Mark T.
通讯作者：
Bedford, Mark T.
影响因子：
56.9
作者：
Lee, KB;Park, SJ;Mrksich, M
通讯作者：
Mrksich, M
影响因子：
64.8
作者：
MULTIGNER, L;GAGNON, J;JOB, D
通讯作者：
JOB, D
DOI：
10.1083/jcb.141.4.993
发表时间：
1998-05-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cole DG;Diener DR;Himelblau AL;Beech PL;Fuster JC;Rosenbaum JL
通讯作者：
Rosenbaum JL
影响因子：
9.2
作者：
Berman, SA;Wilson, NF;Lefebvre, PA
通讯作者：
Lefebvre, PA