Rate of decline of antibody titers to pandemic influenza A (H1N1-2009) by hemagglutination inhibition and virus microneutralization assays in a cohort of seroconverting adults in Singapore.
Rate of decline of antibody titers to pandemic influenza A (H1N1-2009) by hemagglutination inhibition and virus microneutralization assays in a cohort of seroconverting adults in Singapore.
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DOI：
10.1186/1471-2334-14-414
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发表时间：
2014-07-28
影响因子：
3.7
通讯作者：
Chow VT
中科院分区：
文献类型：
--
作者：
Hsu JP;Zhao X;Chen MI;Cook AR;Lee V;Lim WY;Tan L;Barr IG;Jiang L;Tan CL;Phoon MC;Cui L;Lin R;Leo YS;Chow VT
感染后流感抗体滴度的下降速度会影响血清学调查的结果，并可能解释同一毒株的再感染和复发性流行。我们随访了2009年血清发病率研究期间血凝抑制（HI）抗体滴度（增加≥4倍）血清转化为甲型H1N1流感pdm 09的队列。沿着流行前样本和2009年8月至10月期间HI滴度最高的2009年样本（A）外，还通过HI和病毒微量中和（MN）测定法测定了2010年4月和2010年9月获得的另外两份血液样本（B和C）的A（H1N1）pdm 09抗体。我们分析了血清转换后3个时间点的滴度成对平均倍数变化以及HI滴度≥ 40和MN ≥ 160（在我们的检测中与HI滴度40相关）的比例。共有67名参与者每人贡献了3份样本。从2009年最高HI滴度到2010年最后一个样本，2名参与者显示滴度增加（HI和MN），而63名（94%）和49名（73%）分别显示HI和MN滴度降低。两种测定法的滴度均显著降低; 2009年，70.8%和72.3%的受试者的HI和MN滴度≥ 40和≥ 160，截至2010年9月，这些百分比分别降至13.9%和36.9%。在6名55岁及以上的受试者中，下降幅度显著大于55岁以下的受试者，因此在最终样本中，没有老年人的HI滴度≥ 40，MN滴度≥ 160。由于滴度的这种下降，发现在样品A中HI血清转化的65名参与者中只有23名（35%）在流行前样品和样品C之间发生血清转化，而在样品A中MN血清转化的59名参与者中有53名（90%）发生血清转化。我们观察到HI血清转换后1年滴度明显降低，MN降低程度较小。我们的研究结果对再感染、复发性流行病、疫苗接种策略以及通过血清转换测量感染率的队列研究具有意义。本文的在线版本（doi：10.1186/1471-2334-14-414）包含补充材料，可供授权用户使用。
The rate of decline of antibody titers to influenza following infection can affect results of serological surveys, and may explain re-infection and recurrent epidemics by the same strain. We followed up a cohort who seroconverted on hemagglutination inhibition (HI) antibody titers (≥4-fold increase) to pandemic influenza A(H1N1)pdm09 during a seroincidence study in 2009. Along with the pre-epidemic sample, and the sample from 2009 with the highest HI titer between August and October 2009 (A), two additional blood samples obtained in April 2010 and September 2010 (B and C) were assayed for antibodies to A(H1N1)pdm09 by both HI and virus microneutralization (MN) assays. We analyzed pair-wise mean-fold change in titers and the proportion with HI titers ≥ 40 and MN ≥ 160 (which correlated with a HI titer of 40 in our assays) at the 3 time-points following seroconversion. A total of 67 participants contributed 3 samples each. From the highest HI titer in 2009 to the last sample in 2010, 2 participants showed increase in titers (by HI and MN), while 63 (94%) and 49 (73%) had reduction in HI and MN titers, respectively. Titers by both assays decreased significantly; while 70.8% and 72.3% of subjects had titers of ≥ 40 and ≥ 160 by HI and MN in 2009, these percentages decreased to 13.9% and 36.9% by September 2010. In 6 participants aged 55 years and older, the decrease was significantly greater than in those aged below 55, so that none of the elderly had HI titers ≥ 40 nor MN titers ≥ 160 by the final sample. Due to this decline in titers, only 23 (35%) of the 65 participants who seroconverted on HI in sample A were found to seroconvert between the pre-epidemic sample and sample C, compared to 53 (90%) of the 59 who seroconverted on MN on Sample A. We observed marked reduction in titers 1 year after seroconversion by HI, and to a lesser extent by MN. Our findings have implications for re-infections, recurrent epidemics, vaccination strategies, and for cohort studies measuring infection rates by seroconversion. The online version of this article (doi:10.1186/1471-2334-14-414) contains supplementary material, which is available to authorized users.
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