Cellular arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms.
Cellular arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms.
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DOI：
10.1371/journal.pbio.3002205
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发表时间：
2024-02
期刊：
影响因子：
9.8
通讯作者：
Dietrich, Lars E. P.
中科院分区：
文献类型：
--
作者：
Dayton, Hannah;Kiss, Julie;Wei, Mian;Chauhan, Shradha;LaMarre, Emily;Cornell, William Cole;Morgan, Chase J.;Janakiraman, Anuradha;Min, Wei;Tomer, Raju;Price-Whelan, Alexa;Nirody, Jasmine A.;Dietrich, Lars E. P.
细胞必须获得生存所需的资源，而多细胞结构的解剖结构会影响这种获取。在多种多样的多细胞真核生物中，资源是由内部管道提供的，这些管道允许物质比通过扩散更容易地穿过组织。在多细胞结构中生长的微生物，称为生物膜，也受到对资源的差异性访问的影响，我们假设这受到细胞物理排列的影响。在这项研究中，我们研究了由致病性细菌铜绿假单胞菌形成的生物膜的显微解剖，发现克隆细胞形成的条纹是纵向包装在大部分成熟的生物膜的深度。我们确定了突变体，包括那些有缺陷的菌毛功能和O-抗原附着，这表明改变纵向包装表型。与细胞排列影响对生物膜内资源的获取的概念一致，我们发现，虽然野生型显示出测试底物在整个深度上的均匀分布，但突变体显示出底物在生物膜边界处的积累。此外，我们发现生物膜内改变的细胞排列影响代谢活性的定位、驻留细胞的存活以及亚群对抗生素治疗的敏感性。我们的观察提供了深入了解细胞功能，决定生物膜的微观解剖，生理分化和药物敏感性的后果。细胞必须获得生存所需的资源，而多细胞结构的解剖结构会影响这种获取。这项研究表明，生物膜中的细菌沿沿着氧梯度分裂;它们的端到端模式影响分子如何通过生物膜移动，从而影响生物膜亚区的生理和药物敏感性。
Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse multicellular eukaryotes, resources are provided by internal conduits that allow substances to travel more readily through tissue than they would via diffusion. Microbes growing in multicellular structures, called biofilms, are also affected by differential access to resources and we hypothesized that this is influenced by the physical arrangement of the cells. In this study, we examined the microanatomy of biofilms formed by the pathogenic bacterium Pseudomonas aeruginosa and discovered that clonal cells form striations that are packed lengthwise across most of a mature biofilm’s depth. We identified mutants, including those defective in pilus function and in O-antigen attachment, that show alterations to this lengthwise packing phenotype. Consistent with the notion that cellular arrangement affects access to resources within the biofilm, we found that while the wild type shows even distribution of tested substrates across depth, the mutants show accumulation of substrates at the biofilm boundaries. Furthermore, we found that altered cellular arrangement within biofilms affects the localization of metabolic activity, the survival of resident cells, and the susceptibility of subpopulations to antibiotic treatment. Our observations provide insight into cellular features that determine biofilm microanatomy, with consequences for physiological differentiation and drug sensitivity. Cells must access resources to survive, and the anatomy of multicellular structures influences this access. This study shows that bacteria in biofilms divide along oxygen gradients; their end-to-end patterning affects how molecules move through the biofilm, and therefore the physiology and drug susceptibility of biofilm subzones.
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影响因子：
4.6
作者：
Ghanbari A;Dehghany J;Schwebs T;Müsken M;Häussler S;Meyer-Hermann M
通讯作者：
Meyer-Hermann M
影响因子：
11
作者：
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作者：
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Losick, Richard
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通讯作者：
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