Preparation of a Cu(II)-PVA/PA6 composite nanofibrous membrane for enzyme immobilization.
Preparation of a Cu(II)-PVA/PA6 composite nanofibrous membrane for enzyme immobilization.
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制备Cu（II）-PVA/PA6复合纳米纤维膜以固定酶。
DOI：
10.3390/ijms131012734
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发表时间：
2012-10-05
影响因子：
5.6
通讯作者：
Wei A
中科院分区：
文献类型：
--
作者：
Feng Q;Tang B;Wei Q;Hou D;Bi S;Wei A
采用静电纺丝法制备了PVA/PA 6复合纳米纤维。通过PVA/PA 6复合纳米纤维与Cu ~（2+）溶液反应制备Cu（II）-PVA/PA 6金属螯合纳米纤维，并将其用作过氧化氢酶的固定化载体。实验结果表明，PVA/PA 6复合纳米纤维对Cu 2+离子具有良好的螯合能力，且在与Cu 2+溶液的反应过程中，纳米纤维的结构稳定。采用Langmuir等温吸附模型研究了Cu（II）在PVA/PA 6复合纳米纤维上的吸附行为。Cu（II）的最大配位量（qm）为3.731 mmol/g（干纤维），结合常数（KI）为0.0593 L/mmol。动力学参数进行了分析固定化和游离过氧化氢酶。固定化过氧化氢酶的Vmax（3774 μmol/mg·min）小于游离过氧化氢酶的Vmax（4878 μmol/mg·min），但Km值较大。固定化过氧化氢酶的耐酸碱性和耐温性均优于游离酶，且固定化过氧化氢酶的贮存稳定性和重复使用性均得到显著提高。游离和固定化过氧化氢酶的半衰期分别为8天和24天。
PVA/PA6 composite nanofibers were formed by electrospinning. Cu(II)-PVA/PA6 metal chelated nanofibers, prepared by the reaction between PVA/PA6 composite nanofibers and Cu2+ solution, were used as the support for catalase immobilization. The result of the experiments showed that PVA/PA6 composite nanofibers had an excellent chelation capacity for Cu2+ ions, and the structures of nanofibers were stable during the reaction with Cu2+ solution. The adsorption of Cu(II) onto PVA/PA6 composite nanofibers was studied by the Langmuir isothermal adsorption model. The maximum amount of coordinated Cu(II) (qm) was 3.731 mmol/g (dry fiber), and the binding constant (Kl) was 0.0593 L/mmol. Kinetic parameters were analyzed for both immobilized and free catalases. The value of Vmax (3774 μmol/mg·min) for the immobilized catalases was smaller than that of the free catalases (4878 μmol/mg·min), while the Km for the immobilized catalases was larger. The immobilized catalases showed better resistance to pH and temperature than that of free form, and the storage stabilities, reusability of immobilized catalases were significantly improved. The half-lives of free and immobilized catalases were 8 days and 24 days, respectively.
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影响因子：
3
作者：
Feng, Quan;Xia, Xin;Wei, Anjing
通讯作者：
Wei, Anjing
DOI：
10.3390/s101109963
发表时间：
2010
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Ling YP;Heng LY
通讯作者：
Heng LY
影响因子：
--
作者：
Akgol, Sinan;Oztuerk, Nevra;Denizli, Adil
通讯作者：
Denizli, Adil
影响因子：
--
作者：
Dincer, Ayse;Telefoncu, Azmi
通讯作者：
Telefoncu, Azmi
影响因子：
8.8
作者：
Cetinus, Senay Akkus;Sahin, Ebru;Saraydin, Dursun
通讯作者：
Saraydin, Dursun