Prochlorococcus extracellular vesicles: molecular composition and adsorption to diverse microbes.

Prochlorococcus extracellular vesicles: molecular composition and adsorption to diverse microbes.
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细胞外囊泡:分子组成和吸附到多种微生物上。

DOI:
10.1111/1462-2920.15834
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发表时间:
2022-01
影响因子:
5.1
通讯作者:
Chisholm, Sallie W.
Chisholm, Sallie W.
中科院分区:
生物学2区
文献类型:
--
作者:
Biller, Steven J.;Lundeen, Rachel A.;Hmelo, Laura R.;Becker, Kevin W.;Arellano, Aldo A.;Dooley, Keven;Heal, Katherine R.;Carlson, Laura T.;Van Mooy, Benjamin A. S.;Ingalls, Anitra E.;Chisholm, Sallie W.

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细胞外囊泡是小的(直径约50-200 nm)膜结合结构,由来自所有生命领域的细胞释放。虽然囊泡在海洋中很丰富,但它们的功能,无论是对细胞本身还是对新兴生态系统,都仍然是一个谜。为了更好地表征这些颗粒-确定功能的先决条件-我们分析了海洋蓝藻原绿球藻产生的囊泡的脂质,蛋白质和代谢产物含量。我们表明,原绿球藻出口到周围的海水中封闭在离散的囊泡的细胞化合物的多样化阵列。由两种不同菌株产生的囊泡包含一些共同的材料,但也显示出许多菌株特异性差异,反映了囊泡群体内的功能复杂性。囊泡中含有活性酶,表明它们可以介导海洋中的细胞外生物化学反应。我们进一步证明,囊泡从原绿球藻和其他细菌与不同的微生物,包括最丰富的海洋细菌,Pelagibacter。总之,我们的数据指向关于囊泡在海洋生态系统中的功能作用的假设,包括但不限于,可能介导能量和营养转移,催化细胞外生化反应,减轻活性氧的毒性。
Extracellular vesicles are small (~50–200 nm diameter) membrane‐bound structures released by cells from all domains of life. While vesicles are abundant in the oceans, their functions, both for cells themselves and the emergent ecosystem, remain a mystery. To better characterize these particles – a prerequisite for determining function – we analysed the lipid, protein, and metabolite content of vesicles produced by the marine cyanobacterium Prochlorococcus. We show that Prochlorococcus exports a diverse array of cellular compounds into the surrounding seawater enclosed within discrete vesicles. Vesicles produced by two different strains contain some materials in common, but also display numerous strain‐specific differences, reflecting functional complexity within vesicle populations. The vesicles contain active enzymes, indicating that they can mediate extracellular biogeochemical reactions in the ocean. We further demonstrate that vesicles from Prochlorococcus and other bacteria associate with diverse microbes including the most abundant marine bacterium, Pelagibacter. Together, our data point toward hypotheses concerning the functional roles of vesicles in marine ecosystems including, but not limited to, possibly mediating energy and nutrient transfers, catalysing extracellular biochemical reactions, and mitigating toxicity of reactive oxygen species.
DOI: 10.1038/s41598-017-06220-0
发表时间: 2017-07-19
期刊: Scientific reports
影响因子: 4.6
作者:
Tranchida F;Rakotoniaina Z;Shintu L;Tchiakpe L;Deyris V;Yemloul M;Stocker P;Vidal N;Rimet O;Hiol A;Caldarelli S
通讯作者: Caldarelli S
DOI: 10.1038/nrmicro3480
发表时间: 2015-10
期刊: Nature reviews. Microbiology
影响因子: --
作者:
Brown L;Wolf JM;Prados-Rosales R;Casadevall A
通讯作者: Casadevall A
DOI: 10.1038/ismej.2012.122
发表时间: 2013-03-01
期刊: ISME JOURNAL
影响因子: 11
作者:
Carini, Paul;Steindler, Laura;Giovannoni, Stephen J.
通讯作者: Giovannoni, Stephen J.
DOI: 10.1038/ismej.2016.134
发表时间: 2017-02-01
期刊: ISME JOURNAL
影响因子: 11
作者:
Biller, Steven J.;McDaniel, Lauren D.;Chisholm, Sallie W.
通讯作者: Chisholm, Sallie W.
DOI: 10.1021/acs.analchem.6b01260
发表时间: 2016-07-19
影响因子: 7.4
作者:
Collins, James R.;Edwards, Bethanie R.;Van Mooy, Benjamin A. S.
通讯作者: Van Mooy, Benjamin A. S.