Simultaneous measurement of sleep and feeding in individual Drosophila.
Simultaneous measurement of sleep and feeding in individual Drosophila.
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DOI：
10.1038/nprot.2017.096
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发表时间：
2017-11
期刊：
影响因子：
14.8
通讯作者：
Ja WW
中科院分区：
文献类型：
--
作者：
Murphy KR;Park JH;Huber R;Ja WW
Drosophila is widely used for the dissection of genetic and neuronal mechanisms of behavior. Recently, flies have emerged as a model for investigating the regulation of feeding and sleep. Although typically studied in isolation, increasing evidence points to a fundamental connection between these behaviors. Thus, a system for measuring sleep and feeding simultaneously in a single integrated system is important for interpreting behavioral shifts of either state. Here, we describe the construction and use of the Activity Recording CAFE (ARC), a machine-vision based system for the integrated measurement of sleep and feeding in individual Drosophila. Flies feed on liquid food in a microcapillary and consumption is measured by automated tracking of the liquid meniscus over time. Sleep measurements are obtained from positional tracking of the animals and arousal threshold can be determined by vibrational stimulus response. Using this system, a single computer and experimenter can track diverse behaviors from up to 60 individual flies in a single integrated system. The ARC is efficiently assembled with minimal training and each experiment can be run for up to ~7 days, with a total setup and breakdown time of about 2 hours.
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DOI：
10.1073/pnas.0702726104
发表时间：
2007-05-15
影响因子：
11.1
作者：
Ja, William W.;Carvalho, Gil B.;Benzer, Seymour
通讯作者：
Benzer, Seymour
睡眠通过阻止多巴胺神经元介导的遗忘来促进记忆。
影响因子：
64.5
作者：
Berry JA;Cervantes-Sandoval I;Chakraborty M;Davis RL
通讯作者：
Davis RL
DOI：
10.1523/jneurosci.0061-13.2013
发表时间：
2013-04-17
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
van Alphen B;Yap MH;Kirszenblat L;Kottler B;van Swinderen B
通讯作者：
van Swinderen B
影响因子：
3.7
作者：
Donelson NC;Kim EZ;Slawson JB;Vecsey CG;Huber R;Griffith LC
通讯作者：
Griffith LC
影响因子：
48
作者：
Deshpande, Sonali A.;Carvalho, Gil B.;Amador, Ariadna;Phillips, Angela M.;Hoxha, Sany;Lizotte, Keith J.;Ja, William W.
通讯作者：
Ja, William W.